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Loading Controls

Loading Controls

 

Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples.

Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.

Collection Menu

Catalog #1: bsm-33042M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33042M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33042M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33042M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33042M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33042M-Cy5.5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-0917M-Cy3

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy3

Catalog #1: bsm-0917M-Cy5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5

Catalog #1: bsm-0917M-Cy7

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy7

Catalog #1: bsm-0917M-FITC

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: FITC

Catalog #1: bsm-0917M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: HRP

Catalog #1: bsm-0917M-Cy5.5

Applications: WB, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Cy5.5

Catalog #1: bsm-33042M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-0917M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

HeLa cell lysates; probed with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
K562 cell lysates; probed with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, unconjugated (bsm-33042M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Histone H3\/HIST3H3 (3G1) Monoclonal Antibody, Unconjugated (bsm-33042M)at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-33042M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37\u00b0C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37\u00b0C
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
MCF-7 cells were labeled with BrdU (10\u03bcM) and stained with mouse monoclonal antibody against BrdU with dilution at 1:100 and 1:50. 2nd antibody without primary antibody was used as control included here. Fluorescent signals were detected with both 1:100 and 1:50 primary antibody dilution in both MCF-7 it shows nucleus staining. Scale bar= 20 \u03bcm.
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (BrdU (A7)) Monoclonal Antibody, Unconjugated (bsm-0917M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

Catalog #1: bsm-0917M

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Others

Conjugate: Unconjugated

Catalog #1: bsm-33235M-RBITC

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: RBITC

Catalog #1: bsm-33235M-PerCP-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PerCP-Cy7

Catalog #1: bsm-33235M-PerCP-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-33235M-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy5.5

Catalog #1: bsm-33235M-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy5

Catalog #1: bsm-33235M-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy7

Catalog #1: bsm-33235M-PE-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE-Cy3

Catalog #1: bsm-33235M-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC-Cy7

Catalog #1: bsm-33235M-APC-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC-Cy5.5

Catalog #1: bsm-33235M-APC-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC-Cy5

Catalog #1: bsm-33235M-PerCP

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PerCP

Catalog #1: bsm-33235M-PE

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: PE

Catalog #1: bsm-33033M-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Pig

Conjugate: PE

Catalog #1: bsm-33235M-APC

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: APC

Catalog #1: bsm-33235M-FITC

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: FITC

Catalog #1: bsm-33235M-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy7

Catalog #1: bsm-33235M-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy5.5

Catalog #1: bsm-33235M-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy5

Catalog #1: bsm-33235M-Cy3

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Cy3

Catalog #1: bsm-33235M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: HRP

Catalog #1: bsm-0945M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-0945M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-0978M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: HRP

Catalog #1: bsm-33035M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33035M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33035M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33035M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33035M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33033M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: HRP