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Loading Controls

Loading Controls

 

Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples.

Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.



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Catalog #1: bsm-33034m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33034m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0945m-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5.5

Catalog #1: bsm-33042m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33035m-Cy5.5-100ul

Applications: WB, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33034m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33228m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33243m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33033m-cy5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5

Catalog #1: bsm-33033m-cy7-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy7

Catalog #1: bsm-33033m-Cy5.5-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5.5

Catalog #1: bsm-33033m-fitc-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: FITC

Catalog #1: bsm-33033m-cy3-100ul

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy3

Catalog #1: bsm-33128M-HRP

Applications: WB

Reactivity: Plant

Conjugate: HRP

Catalog #1: bsm-33227M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: COX4I1 Monoclonal Antibody, conjugated (bsm-33037M-Cy3) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Incubation: COX4I1 Monoclonal Antibody, conjugated (bsm-33037M-Cy3) at 1:200 overnight at 4\u00b0C; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33037m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33037m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33037m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33037m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33037m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33037m-Cy5.5-100ul

Applications: WB, IF

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33235m-bf750-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-750

Catalog #1: bsm-33235m-bf680-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-680

Catalog #1: bsm-33235m-bf647-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-647

Catalog #1: bsm-33235m-bf594-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-594

Catalog #1: bsm-33235m-bf555-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-555

Catalog #1: bsm-33235m-bf488-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-488

Catalog #1: bsm-33235m-bf405-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-405

Catalog #1: bsm-33235m-bf350-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: AbBy Fluor-350

Catalog #1: bsm-33235m-biotin-100ul

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Biotin

Catalog #1: bsm-0945m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33042m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33035m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33033m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Biotin

Catalog #1: bsm-33040m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33041m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33034m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33228m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33243m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33128M-Biotin

Applications: WB

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bsm-33227M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33037m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-8770M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Catalog #1: bsm-8772M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta-Actin (1A2) Monoclonal Antibody(bsm-33036M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Actin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with beta-Actin monoclonal antibody, unconjugated (bsm-33036M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33036m-100ul

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with alpha Tubulin (Acetyl Lys40) monoclonal antibody, unconjugated (bsm-33235M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
C6 (R) cells were treated with or without Trichostatin A (500 ng\/ml) for 4 h, Hela (H) cells were treated with or without Trichostatin A (500 ng\/ml) for 4 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with alpha Tubulin (Acetyl Lys40) monoclonal antibody, unconjugated (bsm-33235M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33235m

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Lane 1: NIH\/3T3 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-2006M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-33227m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated