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Loading Controls

Loading Controls

 

Loading control proteins are essential for the accurate interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples.

Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. The loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable.

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Catalog #1: bsm-33041M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bsm-33034M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33036M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Biotin

Catalog #1: bsm-33228M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33243M-Biotin

Applications: WB

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33128M-Biotin

Applications: WB

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bsm-33227M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33037M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-8770M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Catalog #1: bsm-8772M

Applications: WB

Reactivity: Caenorhabditis Elegans

Conjugate: Unconjugated

Catalog #1: bsm-33235M

Applications: WB

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Lane 1: NIH\/3T3 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-2006M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-33227M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33128M

Applications: WB

Reactivity: Plant

Conjugate: Unconjugated

Lane 1: Human serum . Probed with Transferrin monoclonal Antibody, unconjugated (bsm-33243M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin(Serum Loading Control) Monoclonal Antibody(bsm-33243M)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-33243M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33228M

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: U87mg lysates; Lane 2: SW480 lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-33034M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: HL60 lysates;Lane 2: U937 lysates;Lane 3: A431 lysates; Lane 4: SH-SY5Y lysates probed with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3:HL60 cell lysates; Lane 4: SH-SY5Y cell lysates ; Lane 5:Mouse Cerebellum cell lysates;Lane 6:Rat Cerebellum cell lysates;7: Mouse Cerebrum cell lysates;Lane 8: Rat Cerebrum cell lysates probed with Beta tubulin Monoclonal Antibody, Unconjugated (bsm-33034M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33034M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 cell lysates; Lane 2: Jurkat cell lysates; probed with beta I Tubulin (5F7) Monoclonal Antibody, unconjugated (bsm-33041M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with beta 1 Tubulin Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1\uff1aHuman 293T cell lysates; Lane 2: Mouse Cerebellum lysates; Lane 3:Human Jurkat cell lysates probed with beta 1 Tubulin Monoclonal Antibody, Unconjugated (bsm-33041R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HeLa cells were stained with beta 1 Tubulin (5F7) Monoclonal Antibody, Unconjugated(bsm-33041M) at 1:50 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Mouse IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Catalog #1: bsm-33041M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

MOLT-4 cell lysates probed with Lamin B (9C11) Monoclonal Antibody, unconjugated (bsm-33040M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Rat Pancreas tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Rat Thymus tissue lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human Molt-4 cell lysates; Lane 6: Human Hela cell lysates; Lane 7: Human U-2os cell lysates; Lane 8: Human THP-1 cell lysates probed with Lamin B Monoclonal Antibody, Unconjugated (bsm-33040M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33040M

Applications: WB

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: 293T Cell lysates; probed with GAPDH (4F8) Monoclonal Antibody, unconjugated (bsm-33033M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: A431 cell lysates; Lane 2: Hela cell lysates; Lane 3: Jurkat cell lysates; Lane 4: Lovor cell lysates probed with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hyperpituitarism lysates probed with GAPDH (4F8) Monoclonal Antibody, Unconjugated (bsm-33033M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Cerebrum lysates; Lane 5: Mouse Testis lysates; Lane 6: Rat Testis lysates; Lane 7: Mouse Kidney lysates; Lane 8: Human HUVEC cell lysates; Lane 9: Human A549 cell lysates; Lane 10: Human MCF-7 cell lysates probed with GAPDH Monoclonal Antibody, Unconjugated (bsm-33033M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33033M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A549 lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Lung lysates; Lane 2: Mouse Spleen lysates probed with Serum albumin Polyclonal Antibody, Unconjugated (bsm-0945M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0945M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Human HepG2 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Human A549 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: mouse placenta; Lane 2: 293T cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates probed with GAPDH(3E12) Monoclonal Antibody, Unconjugated (bsm-0978M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0978M

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Unconjugated

Lane 1: 293T; Lane 2: NIH\/3T3; Lane 3: mouse muscle lysates probed with beta-Actin (1A2) Monoclonal Antibody (bsm-33036M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lovo cell lysates probed with beta-Actin (1A2) Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: A431 cell lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Lung lysates probed with beta-Actin Monoclonal Antibody, Unconjugated (bsm-33036M ) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33036M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Pig

Conjugate: Unconjugated

NIH\/3T3 cells probed with PCNA (3G1) Monoclonal Antibody, unconjugated (bsm-33035M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
HeLa cell lysate probed with PCNA (3G1) Monoclonal Antibody, unconjugated (bsm-33035M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
A431 cell lysate probed with PCNA (3G1) Monoclonal Antibody, unconjugated (bsm-33035M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n
Paraformaldehyde-fixed, paraffin embedded rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA (3G1) Monoclonal Antibody, Unconjugated (bsm-33035M) at 1:400 overnight at 4\u00b0C, followed by operating according to DAB staining.

Catalog #1: bsm-33035M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
HepG2 cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33037M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated