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Mitochondria Marker Antibodies

 

 

Mitochondria are membrane-bound cell organelles that generate most of the chemical energy needed to power the cell's biochemical reactions. The energy produced by the mitochondria is stored in a small molecule called adenosine triphosphate (ATP).

Our antibodies will help you to stain these mitochondria markers in your samples.

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Lane 1: Mouse heart; Lane 2: Rat heart; Probed with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human liver cancer tissue incubated with COX4 (1C4) Monoclonal Antibody (bsm-52750R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52750R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
HepG2 cell lysates probed with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:20000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 (8D8) Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:2000 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 Monoclonal Antibody, Unconjugated (bsm-33037M) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bsm-33037M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33037M-A350

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-33037M-A488

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-33037M-A555

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-33037M-A594

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-33037M-A647

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-33037M-A680

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-33037M-A750

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-33037M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33037M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33037M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33037M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33037M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33037M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: HRP

HeLa cells probed with Rabbit Anti-COX4 Polyclonal Antibody (bs-R) at 1:20 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG conjugated secondary FITC (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\\n
Validation submitted by One World Lab. MCF-7 cells were stained with COX4 Polyclonal Antibody, Unconjugated\uff0cbs-10257R at 1:50 in PBS and incubated for one hour at room temperature, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Mouse kidney lysates probed with COX4 Polyclonal Antibody, Unconjugated (bs-10257R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-10257R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10257R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-10257R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-10257R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-10257R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-10257R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-10257R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-10257R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-10257R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10257R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-10257R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-10257R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-10257R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-10257R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

MCF-7 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with Cytochrome C (1F10) Monoclonal Antibody (bsm-52050R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52050R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-33193M-A350

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-33193M-A488

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-33193M-A555

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-33193M-A594

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-33193M-A647

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-33193M-A680

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-33193M-A750

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-33193M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-33193M-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bsm-33193M-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bsm-33193M-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bsm-33193M-FITC

Applications: WB, IF(IHC-P)

Reactivity: Human, Rat, Rabbit

Conjugate: FITC

Catalog #1: bsm-33193M-HRP

Applications: WB, IHC-P

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Lane 1: Mouse kidney; Lane 2: Rat kidney; Probed with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C
Paraformaldehyde-fixed and paraffin-embedded Human liver tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with Cytochrome C (8D2) Monoclonal Antibody (bsm-52497R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52497R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0013R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0013R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 488