PAPPA Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAPPA Monoclonal Antibody, Unconjugated(bsm-34258M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAPPA Monoclonal Antibody, Unconjugated(bsm-34258M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Mouse, sp-0024) and DAB (C-0010) staining.

Applications

ELISA
IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-34258m
Product Name	PAPPA Monoclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human PAPPA
Clonality	Monoclonal
Clone #	2H5
Isotype	IgG1
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5069
Swiss Prot	Q13219
Subcellular location	Secreted
Synonyms	ASBABP2; Aspecific BCL2 ARE binding protein 2; Differentially placenta 1 expressed protein; DIPLA1; IGF dependent IGFBP 4 protease; IGF-dependent IGFBP-4 protease; IGFBP 4ase; IGFBP-4ase; IGFBP4ase; Insulin like growth factor dependent IGF binding protein 4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPA; PAPP A; PAPP-A; PAPP1_HUMAN; PAPPA; PAPPA1; Pappalysin 1 precursor; Pappalysin-1; Pregnancy Associated Plasma Protein A; Pregnancy associated plasma protein A pappalysin 1; Pregnancy-associated plasma protein A.
Background	Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF