PR3 Monoclonal Antibody

$4% Paraformaldehyde-fixed HL-60 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PR3) monoclonal Antibody, unconjugated (bsm-43164M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HL-60 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PR3) monoclonal Antibody, unconjugated (bsm-43164M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Applications

IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-43164m
Product Name	PR3 Monoclonal Antibody
Applications	IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human PR3
Clonality	Monoclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M PBS(pH7.4)
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cell membrane
Synonyms	ACPA; AGP 7; AGP7; AGP7 serine proteinase; Azurophil Granule Protein 7; C ANCA antigen; C-ANCA antigen; EC 3.4.21.76; Leukocyte proteinase 3; MBN; MBT WEGENER AUTOANTIGEN; Myeloblastin; Neutrophil proteinase 4; NP 4; NP-4; NP4; P29; PR 3; PR-3; Proteinase3; PRTN 3; Prtn3; PRTN3_HUMAN; Serine proteinase neutrophil Wegener granulomatosis autoantigen; Wegener autoantigen.
Background	Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100