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Monoclonal IHC Antibodies



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Lane 1: Mouse Brain; Lane 2: SK-Br-3 Cells; Lane 3: Rat Lung; Probed with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54193R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ZAP70 monoclonal antibody, unconjugated (bsm-54192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZAP70 Monoclonal Antibody, Unconjugated(bsm-54192R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human lymph nodes ;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZAP70 Monoclonal Antibody, Unconjugated(bsm-54192R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
\uff08Negative control\uff09Paraformaldehyde-fixed, paraffin embedded Human liver ;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZAP70 Monoclonal Antibody, Unconjugated(bsm-54192R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54192r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54107r-100ul

Applications: WB, FCM, IP

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Lane 1: HL-60 Cells; Probed with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Carcinoma tissue incubated with TNFRSF10B (1D8) Monoclonal Antibody (bsm-54027R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-54027R

Applications: WB, FCM, IHC-P, IF(IHC-P), IP

Reactivity: Human

Conjugate: Unconjugated