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Monoclonal IHC Antibodies



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Lane 1: MCF-7; Lane 2: A431; Lane 3: HeLa; Lane 4: HepG2; Lane 5: NIH\/3T3; Lane 6: mouse stomach; Lane 7: mouse liver lysate at 35 \u00b5g per lane. probed with bsm-51216M ENO1 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ENO1 (7C4) Monoclonal Antibody (bsm-51216M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51216M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: WiDr; Lane 2: mouse stomach lysate at 20 \u00b5g per lane, probed with bsm-51214M CDH1 (813CT11.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (813CT11.1.3) Monoclonal Antibody (bsm-51214M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51214M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51201M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

NCCIT cell lysate probed with bsm-51142M SOX2 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: SOX2 Protein Isolate; probed with SOX2 (7C4) Monoclonal Antibody, unconjugated (bsm-51142M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:50 (37\u2103 for 1hr) in A549 cells (green). Cells were counterstained with Alexa Fluor\u00ae 555 (red) conjugated Phalloidin. Cells were fixed in 4% paraformaldehyde for 20min and permeabilized with 0.1% Triton X100\/PBS for 10min.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-51142M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-2313M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat submandibular gland labeled with Mouse Anti-PSA(Pa3) Monoclonal Antibody (bsm-1625M) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Human Prostate Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSA(Pa3) Monoclonal Antibody, Unconjugated (bsm-1625M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PSA(Pa3) Monoclonal Antibody, Unconjugated (bsm-1625M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-1625m-100ul

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1624m-100ul

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1622m-100ul

Applications: WB, ELISA, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal cancer tissue labeled with Anti-WIG 1\/PAG608 (3E4) Monoclonal Antibody, Unconjugated(bsm-0957M) at 1:200 followed by conjugation to the secondary antibody and DAB staining
293T cell lysates probed with PAG608 (3E4) Monoclonal Antibody, Unconjugated (bsm-0957M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat Cerebrum lysates probed with PAG608 (3E4) Monoclonal Antibody, Unconjugated (bsm-0957M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates probed with PAG608 (3E4) Monoclonal Antibody, Unconjugated (bsm-0957M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0957M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-2059M

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Unconjugated

HT29 Cell lysates probed with CEA (5F2) Monoclonal Antibody, unconjugated (bsm-4793M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CEA (5F2) Monoclonal Antibody, Unconjugated (bsm-4793M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-4793M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0940M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated