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Monoclonal IHC Antibodies



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Catalog #1: bsm-33192m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33072M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

A431 cell lysates probed with PNSE (15E2) Monoclonal Antibody, unconjugated (bsm-33072M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
U87MG cell lysates probed with PNSE (15E2) Monoclonal Antibody, unconjugated (bsm-33072M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NSE (15E2) Monoclonal Antibody, Unconjugated (bsm-33072M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NSE (15E2) Monoclonal Antibody, Unconjugated (bsm-33072M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33072M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33048M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: HRP

Catalog #1: bsm-33048M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Pig

Conjugate: Biotin

Catalog #1: bsm-33041m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-33041m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

HeLa cells were stained with beta 1 Tubulin (5F7) Monoclonal Antibody, Unconjugated(bsm-33041M) at 1:50 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Mouse IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
25 ug total protein per lane of various lysates (see on figure) probed with beta I Tubulin monoclonal antibody, unconjugated (bsm-33041M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated(bsm-33041M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta I Tubulin Monoclonal Antibody, Unconjugated(bsm-33041M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33041m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-33187m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

25 ug total protein per lane of various lysates (see on figure) probed with PCNA monoclonal antibody, HRP conjugated (bsm-33035M-HRP) at 1:1000 dilution and 4\u00b0C overnight incubation.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA\/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4\u00b0C, followed by DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA\/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4\u00b0C, followed by DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA\/HRP Monoclonal Antibody, Unconjugated(bsm-33035M-HRP) at 1:200 overnight at 4\u00b0C, followed by DAB (C-0010) staining.

Catalog #1: bsm-33035m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33035m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33293M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bsm-33293M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bsm-33219M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken

Conjugate: HRP

Catalog #1: bsm-51046M

Applications: WB, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51042M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51034M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51029M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51026M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: CCRF-CEM cell lysate (20ug); Lane 2: HeLa cell lysate (20ug) probed with bsm-51009M TBB1 (6A4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB1 (6A4) Monoclonal Antibody, Unconjugated (bs-51009R) at 1:10 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
HepG2 cells stained with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), nuclear staining (blue).

Catalog #1: bsm-51009M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: human brain; Lane 2: Jurkat; Lane 3: mouse brain; Lane 4: mouse lung; Lane 5: rat brain cell lysate at 20 \u00b5g per lane, probed with bsm-51443M PPP2R1B (1496CT356.164.25.226) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PPP2R1B (6C6) Monoclonal Antibody (bsm-51443M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51443M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; Lane 2: SK-BR-3 Cell lysates; Lane 3: C2C12 Cell lysates; probed with CAPN2 (1C1) Monoclonal Antibody, unconjugated (bsm-51429M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CAPN2 (1C1) Monoclonal Antibody (bsm-51429M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Flow cytometric analysis of U-87 MG cells using bsm-51429M CAPN2 Antibody (green) compared to mouse IgG2b isotype control (blue). bsm-51429M was diluted at 1:100 dilution. Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Catalog #1: bsm-51429M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: HepG2 Cell lysates; Lane 2: Rat Liver lysates; Lane 3: MCF-7 Cell lysates; Lane 4: NIH\/3T3 Cell lysates; probed with PHB (12C6) Monoclonal Antibody, unconjugated (bsm-51395M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PHB (12C6) Monoclonal Antibody (bsm-51395M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51395M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse brain lysates; Lane 2: HeLa Cell lysates; Lane 3: Y79 Cell lysates; Lane 4: CEM Cell lysates; Lane 5: PC-12 Cell lysates; probed with TBB5 (7D3) Monoclonal Antibody, unconjugated (bsm-51387M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
A549 cells stained with TBB5 (7D3) Monoclonal Antibody (bsm-51387M) at 1:25 dilution, followed by Alexa Fluor 488-conjugated secondary antibody (green), cytoplasmic actin (red).

Catalog #1: bsm-51387M

Applications: WB, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HeLa; Lane 2: Jurkat; Lane 3: C2C12; Lane 4: PC-12; Lane 5: rat brain; Lane 6: 293T\/17 lysates (20\u00b5g) probed with bsm-51322M RAB11A (6C8) Monoclonal Antibody at 1:4000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAB11A (6C8) Monoclonal Antibody (bsm-51322M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51322M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with SODM (9C3) Monoclonal Antibody (bsm-51319M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: human brain lysate; Lane 2: Hela cell lysate; Lane 3: MCF-7 cell lysate; Lane 4: NIH\/3T3 cell lysate; Lane 5: SH-SY5Y cell lysate; probed with SODM (9C3) Polyclonal Antibody, unconjugated (bsm-51319M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51319M

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: HeLa Cell lysates; Lane 3: K562 Cell lysates; Lane 4: MCF-7 Cell lysates; Lane 5: NIH\/3T3 Cell lysates; probed with CDK4 (9C3) Monoclonal Antibody, unconjugated (bsm-51314M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDK4 (9C3) Monoclonal Antibody (bsm-51314M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining

Catalog #1: bsm-51314M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS cells labeled with HINT1 (5C3) Monoclonal Antibody (bsm-51304M) at 1\/25 dilution, followed by secondary antibody (green), cytoplasmic actin staining (red), and nuclear counter stain with DAPI (blue).
Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with HINT1 (5C3) Monoclonal Antibody (bsm-51304M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Jurkat cells probed with using HINT1 (5C3) Monoclonal Antibody bsm-51304M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 compared (green) compared to an isotype control of mouse IgG1 (blue).
Lane 1: Jurkat; Lane 2: Raji; Lane 3: HepG2; Lane 4: Rat Brain lysates; Lane 5: Mouse Liver lysates; probed with HINT1 (5C3) Polyclonal Antibody, unconjugated (bsm-51304M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51304M

Applications: WB, FCM, IHC-P, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

ICC analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeled with Zap70 (4C2) Monoclonal Antibody (bsm-51298M) at 1:25 dilution, followed by secondary antibody incubation. Immunofluorescence image shows cytoplasm staining of Zap70 (green), actin (red), and nuclear counter stain with DAPI (blue).
Lane 1: Jurkat Cell lysates; Lane 2: MOLT-4 Cell lysates; Lane 3: mouse thymus Cell lysates; probed with Zap70 (4C2) Monoclonal Antibody, unconjugated (bsm-51298M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Jurkat cells probed with using Zap70 (4C2) Monoclonal Antibody, bsm-51298M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:400 (green) compared to an isotype control of mouse IgG2a (blue).

Catalog #1: bsm-51298M

Applications: WB, FCM, IHC-P, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51296M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: THP-1 Cell lysates; Lane 2: mouse liver lysates; Lane 3: mouse testis lysates; probed with ATG3 (7C2) Monoclonal Antibody, unconjugated (bsm-51284M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51284M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51284M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skeletal muscle tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ATG3 (7C2) Monoclonal Antibody (bsm-51285M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51284M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human skin tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: A431; Lane 2: NIH\/3T3; Lane 3: C6 cell lysate probed with RAC1 (13C4) Monoclonal Antibody (bsm-51282M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-51282M

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PIN1 monoclonal antibody, unconjugated (bsm-51230M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51230m-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HeLa; Lane 2: HepG2; Lane 3: HIN\/3T3 cell lysate probed with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

Catalog #1: bsm-51220M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: A431; Lane 3: HeLa; Lane 4: HepG2; Lane 5: NIH\/3T3; Lane 6: mouse stomach; Lane 7: mouse liver lysate at 35 \u00b5g per lane. probed with bsm-51216M ENO1 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ENO1 (7C4) Monoclonal Antibody (bsm-51216M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51216M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: WiDr; Lane 2: mouse stomach lysate at 20 \u00b5g per lane, probed with bsm-51214M CDH1 (813CT11.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (813CT11.1.3) Monoclonal Antibody (bsm-51214M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51214M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0387m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0933M-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0933M-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-0917m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin