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Monoclonal IHC Antibodies



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Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (CDX2 (3G7)) Monoclonal Antibody, Unconjugated (bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with CDX2 Monoclonal Antibody, Unconjugated(bsm-52348R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52348r

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH\/3T3 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-2006M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Rat muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytochrome C (6B10) Monoclonal Antibody, Unconjugated (bsm-33193M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33193m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-2279M

Applications: ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-4713m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: HRP

Catalog #1: bsm-4713m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Biotin

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-4713M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-4713M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining.

Catalog #1: bsm-4713m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Human, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #1: bsm-33193m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bsm-33193m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-2279M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bsm-2279M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-1625M

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated