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Monoclonal IHC Antibodies



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25 ug total protein per lane of various lysates (see on figure) probed with GART monoclonal antibody, unconjugated (bsm-52537R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GART Monoclonal Antibody, Unconjugated (bsm-52537R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GART Monoclonal Antibody, Unconjugated (bsm-52537R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GART Monoclonal Antibody, Unconjugated (bsm-52537R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52537r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Topoisomerase I Monoclonal Antibody, Unconjugated (bsm-52531R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Topoisomerase I Monoclonal Antibody, Unconjugated (bsm-52531R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Topoisomerase I Monoclonal Antibody, Unconjugated (bsm-52531R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Topoisomerase I Monoclonal Antibody, Unconjugated (bsm-52531R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52531r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with PRP19 (7H10) Monoclonal Antibody (bsm-52524R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Jurkat cell lysate probed with PRP19 (7H10) Monoclonal Antibody (bsm-52524R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PRP19 (7H10) Monoclonal Antibody (bsm-52524R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PRP19 (7H10) Monoclonal Antibody (bsm-52524R) at 1:100 in B16-F1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52524R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with alpha 1 Catenin (3D10) Monoclonal Antibody (bsm-52516R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Hela Cells; Lane 2: HepG2 Cells; Lane 3: A431 Cells; Probed with alpha 1 Catenin (3D10) Monoclonal Antibody (bsm-52516R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with alpha 1 Catenin (3D10) Monoclonal Antibody (bsm-52516R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse uterus tissue incubated with alpha 1 Catenin (3D10) Monoclonal Antibody (bsm-52516R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52516R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Jurkat Cells; Lane 2: Hela Cells; Lane 3: NIH\/3T3 Cells; Probed with HNRNPK (8G5) Monoclonal Antibody (bsm-52510RR) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with hnRNP K (8G5) Monoclonal Antibody (bsm-52510R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52510R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of A549 cells with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
MCF-7 cell lysate probed with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with AKT1\/2\/3 (35B2) Monoclonal Antibody (bsm-52468R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52468R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse brain; Lane 2: Mouse kidney; Probed with beta \u2161 Tubulin (48D2) Monoclonal Antibody (bsm-52467R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with beta \u2161 Tubulin (48D2) Monoclonal Antibody (bsm-52467R) at 1:100 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with beta \u2161 Tubulin (48D2) Monoclonal Antibody (bsm-52467R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Brian tissue incubated with beta \u2161 Tubulin (48D2) Monoclonal Antibody (bsm-52467R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52467R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5A Monoclonal Antibody, Unconjugated(bsm-52451R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5A Monoclonal Antibody, Unconjugated(bsm-52451R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5A Monoclonal Antibody, Unconjugated(bsm-52451R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with RAB5A Monoclonal Antibody, Unconjugated(bsm-52451R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52451r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SHG-44 cells; Lane 2: Mouse brain; Lane 3: Mouse liver; Lane 4: Mouse skeletal muscle; Probed with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MAP1LC3A (9A7) Monoclonal Antibody (bsm-52450R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52450R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PARP monoclonal antibody, unconjugated (bsm-52433R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PARP Monoclonal Antibody, Unconjugated(bsm-52433R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PARP Monoclonal Antibody, Unconjugated(bsm-52433R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PARP Monoclonal Antibody, Unconjugated(bsm-52433R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.

Catalog #1: bsm-52433r-TR

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: SH-SY-5Y cell; Lane 2: PC-12 cell; Lane 3: SHG-44 cell; Probed with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100 in SHG-44 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with CaMK\u2161 (1H12) Monoclonal Antibody (bsm-52427R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52427R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity:

Conjugate: Unconjugated

Lane 1: Mouse brain; Lane 2: SH-SY-5Y cell; Probed with TrkA (4G1 ) Monoclonal Antibody (bsm-52424R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of SH-SY-5Y cells with TrkA (4G1) Monoclonal Antibody (bsm-52424R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with TrkA (4G1) Monoclonal Antibody (bsm-52424R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TrkA (4G1) Monoclonal Antibody (bsm-52424R) at 1:100 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52424R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5T4 Monoclonal Antibody, Unconjugated (bsm-52422R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with 5T4 Monoclonal Antibody, Unconjugated (bsm-52422R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
25 ug total protein per lane of various lysates (see on figure) probed with 5T4 monoclonal antibody, unconjugated (bsm-52422R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-52422r-TR

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

NIH\/3T3 cell lysate probed with Caldesmon (14G2) Monoclonal Antibody (bsm-52411R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of NIH\/3T3 cells with Caldesmon (14G2) Monoclonal Antibody (bsm-52411R) at a 1:50 dilution (blue) compared with an unlabeled control (cells without incubation with primary antibody; red).
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Caldesmon (14G2) Monoclonal Antibody (bsm-52411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Caldesmon (14G2) Monoclonal Antibody (bsm-52411R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52411R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with Integrin beta 3 monoclonal antibody, unconjugated (bsm-52374R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ITGB3 Monoclonal Antibody, Unconjugated(bsm-52374R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ITGB3 Monoclonal Antibody, Unconjugated(bsm-52374R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ITGB3 Monoclonal Antibody, Unconjugated(bsm-52374R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52374r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with MAP2K1\/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MAP2K1\/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2K1\/2 Monoclonal Antibody, Unconjugated (bsm-52280R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MAP2K1\/2 Monoclonal Antibody, Unconjugated (bsm-52280R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52280r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Untreated CRC whole cell lysates; Lane 2: CRC cells treated with 1.5\u00b5g\/ml Colcemid for 12 hours whole cell lysates; Probed with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4 ) Monoclonal Antibody (bsm-52267R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4) Monoclonal Antibody (bsm-52267R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon cancer tissue incubated with Phospho-Histone H1.3 (T17)+Histone H1.4 (T17) (3A4) Monoclonal Antibody (bsm-52267R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52267R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Rat Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of C2C12 cells with Calsequestrin-1 (8G1) Monoclonal Antibody (bsm-54285R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Catalog #1: bsm-54285R

Applications: FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: SK-BR-3 Cells; Lane 2: A549 Cells; Lane 3: Mouse Lung; Probed with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cytochrome P450 Oxidoreductase (4F7) Monoclonal Antibody (bsm-54281R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54281R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Rat Kidney; Lane 2: Human Kidney; Probed with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Kidney tissue incubated with NPHS2 (1A5) Monoclonal Antibody (bsm-54280R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54280r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human Kidney; Lane 2: MCF-7 Cells; Probed with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated with PCK2 (12C5) Monoclonal Antibody (bsm-54275R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54275R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T Cells; Lane 2: Hela Cells; Probed with Exportin-1 (2C4) Monoclonal Antibody (bsm-54273R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Exportin-1 (2C4) Monoclonal Antibody (bsm-54273R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Exportin-1 (2C4) Monoclonal Antibody (bsm-54273R) at 1:100 in SiHa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Exportin-1 (2C4) Monoclonal Antibody (bsm-54273R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54273R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Brain; Lane 2: Mouse Brain; Lane 3: Rat Brain; Lane 4: 293 Cells; Probed with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100 in 293T cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Rat Brain tissue incubated with Creatine Kinase B (1D8) Monoclonal Antibody (bsm-54297R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54297R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Testis; Lane 2: PC-3M Cells; Lane 3: A549 Cells; Probed with COPS5 (5H4) Monoclonal Antibody (bsm-54269R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with COPS5 (5H4) Monoclonal Antibody (bsm-54269R) at 1:200 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with COPS5 (5H4) Monoclonal Antibody (bsm-54269R) at 1:200 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with COPS5 (5H4) Monoclonal Antibody (bsm-54269R) at 1:200 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54269R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with VPS26A (24D1) Monoclonal Antibody (bsm-54267R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with VPS26A (24D1) Monoclonal Antibody (bsm-54267R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Kidney tissue incubated with VPS26A (24D1) Monoclonal Antibody (bsm-54267R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Rat Kidney; Lane 2: A431 Cells; Probed with VPS26A (24D1) Monoclonal Antibody (bsm-54267R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-54267R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cells; Probed with SET (2A1) Monoclonal Antibody (bsm-54264R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SET (2A1) Monoclonal Antibody (bsm-54264R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SET (2A1) Monoclonal Antibody (bsm-54264R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SET (2A1) Monoclonal Antibody (bsm-54264R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54264r-100ul

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Daudi Cells; Lane 2: A431 Cells; Lane 3: MCF-7 Cells; Probed with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Testis tissue incubated with DNA Ligase I (3G2) Monoclonal Antibody (bsm-54261R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54261R

Applications: WB, IHC-P

Reactivity: Human, Rat

Conjugate: Unconjugated

Lane 1: NIH-3T3 Cells; Lane 2: SH-SY5Y Cells; Lane 3: K562 Cells; Probed with HNRNPU (1F6) Monoclonal Antibody (bsm-54257R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with HNRNPU (1F6) Monoclonal Antibody (bsm-54257R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with HNRNPU (1F6) Monoclonal Antibody (bsm-54257R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with HNRNPU (1F6) Monoclonal Antibody (bsm-54257R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54257R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cells; Probed with RNF2 (1F4) Monoclonal Antibody (bsm-54254R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated with RNF2 (1F4) Monoclonal Antibody (bsm-54254R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54254R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Brain; Lane 2: SiHa Cells; Lane 3: K562 Cells; Probed with Septin 2 (8C3) Monoclonal Antibody (bsm-54252R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Septin 2 (8C3) Monoclonal Antibody (bsm-54252R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Septin 2 (8C3) Monoclonal Antibody (bsm-54252R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Septin 2 (8C3) Monoclonal Antibody (bsm-54252R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54252R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: PC-3M Cells; Probed with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Fructose 6 Phosphate Kinase (14C3) Monoclonal Antibody (bsm-54246R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54245R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Testis; Lane 2: SiHa Cells; Lane 3: SK-BR-3 Cells; Lane 4: PC-3M Cells; Probed with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CSE1L (4A9) Monoclonal Antibody (bsm-54238R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54238R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATPB Monoclonal Antibody, Unconjugated(bsm-52904R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATPB Monoclonal Antibody, Unconjugated(bsm-52904R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATPB Monoclonal Antibody, Unconjugated(bsm-52904R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ATPB Monoclonal Antibody, Unconjugated(bsm-52904R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52904r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of sh-sy5ycells with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: A431 lysates; Lane 2: Mouse lung lysates; Probed with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:100 in HeLa cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with S100 alpha 6 (7A3) Monoclonal Antibody (bsm-52810R) at 1:300 in A431 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52810R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of MCF7 cells with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 lysates; Lane 2: HepG2 lysates; Line 3: A431 lysates; Probed with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n
IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with 67kDa Laminin Receptor (8C5) Monoclonal Antibody (bsm-52792R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52792R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of NIH\/3T3 cells with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HUVEC cells lysates; Probed with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52790R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of NIH\/3T3 cells with STAT 5A+B (4C4) Monoclonal Antibody (bsm-52732R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 Cells; Lane 2: Jurkat Cells; Lane 3: PC-12 Cells; Probed with STAT5A + STAT5B (4C4) Monoclonal Antibody (bsm-52732R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with STAT5A + STAT5B (4C4) Monoclonal Antibody (bsm-52732R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with STAT5A + STAT5B (4C4) Monoclonal Antibody (bsm-52732R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52732R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells probed with APC6 (7C2) Monoclonal Antibody (bsm-52699R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with APC6 (7C2) Monoclonal Antibody (bsm-52699R) at 1:100 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52699R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PAX6 monoclonal antibody, unconjugated (bsm-52682R) at 1:3000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX6 Monoclonal Antibody, Unconjugated(bsm-52682R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX6 Monoclonal Antibody, Unconjugated(bsm-52682R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX6 Monoclonal Antibody, Unconjugated(bsm-52682R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52682r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: U937 Cells; Lane 2: THP-1 Cells; Probed with JunB (2C1) Monoclonal Antibody (bsm-52681R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with JunB (2C1) Monoclonal Antibody (bsm-52681R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with JunB (2C1) Monoclonal Antibody (bsm-52681R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52681R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cells; Lane 2:HepG2 Cells; Probed with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52677R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: NIH-3T3 Cells; Lane 3: Rat Kidney; Lane 4: Mouse Testis; Probed with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54207r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Lane 1: Mouse Liver; Probed with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54205R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH-3T3 Cells; Lane 2: Hela Cells; Lane 3: Rat Brain; Lane 4: Mouse Testis; Probed with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54204R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with NF-H monoclonal antibody, unconjugated (bsm-52851R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-H Monoclonal Antibody, Unconjugated(bsm-52851R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-H Monoclonal Antibody, Unconjugated(bsm-52851R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-H Monoclonal Antibody, Unconjugated(bsm-52851R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52851r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52820r-100ul

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52812r-100ul

Applications: WB, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293 Cells; Lane 2: MCF-7 Cells; Lane 3: Hela Cells; Probed with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:300 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52644R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated