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Lane 1: 293T Cells; Lane 2: 293 Cells; Probed with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human colon cancer tissue incubated with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with EZH2 (9B6) Monoclonal Antibody (bsm-52743R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52743R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of NIH\/3T3 cells with STAT 5A+B (4C4) Monoclonal Antibody (bsm-52732R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 Cells; Lane 2: Jurkat Cells; Lane 3: PC-12 Cells; Probed with STAT5A + STAT5B (4C4) Monoclonal Antibody (bsm-52732R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with STAT5A + STAT5B (4C4) Monoclonal Antibody (bsm-52732R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with STAT5A + STAT5B (4C4) Monoclonal Antibody (bsm-52732R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52732R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Hela cells probed with APC6 (7C2) Monoclonal Antibody (bsm-52699R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with APC6 (7C2) Monoclonal Antibody (bsm-52699R) at 1:100 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52699R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PAX6 monoclonal antibody, unconjugated (bsm-52682R) at 1:3000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX6 Monoclonal Antibody, Unconjugated(bsm-52682R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX6 Monoclonal Antibody, Unconjugated(bsm-52682R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PAX6 Monoclonal Antibody, Unconjugated(bsm-52682R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52682r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: U937 Cells; Lane 2: THP-1 Cells; Probed with JunB (2C1) Monoclonal Antibody (bsm-52681R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with JunB (2C1) Monoclonal Antibody (bsm-52681R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with JunB (2C1) Monoclonal Antibody (bsm-52681R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52681R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cells; Lane 2:HepG2 Cells; Probed with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with Stromal interaction molecule 1 (7C4) Monoclonal Antibody (bsm-52677R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52677R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

IF(ICC) staining with TYRP1 (5C2) Monoclonal Antibody (bsm-54216R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TYRP1 (5C2) Monoclonal Antibody (bsm-54216R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Lane 1: Human Melanoma; Probed with TYRP1 (5C2) Monoclonal Antibody (bsm-54216R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with TYRP1 (5C2) Monoclonal Antibody (bsm-54216R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54216r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Jurkat Cells; Lane 2: Mouse Brain; Probed with ACTL6A (6T1) Monoclonal Antibody (bsm-54210R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with ACTL6A (6T1) Monoclonal Antibody (bsm-54210R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ACTL6A (6T1) Monoclonal Antibody (bsm-54210R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with ACTL6A (6T1) Monoclonal Antibody (bsm-54210R)at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54210R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: NIH-3T3 Cells; Lane 3: Rat Kidney; Lane 4: Mouse Testis; Probed with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with NCK1 (7C2) Monoclonal Antibody (bsm-54207R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54207r

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Lane 1: Hela Cells; Probed with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with Cullin 4A (3C3) Monoclonal Antibody (bsm-54206R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54206R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Mouse Liver; Probed with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SLC1A1 (4C3) Monoclonal Antibody (bsm-54205R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54205R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: NIH-3T3 Cells; Lane 2: Hela Cells; Lane 3: Rat Brain; Lane 4: Mouse Testis; Probed with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with RBBP4 (4C5) Monoclonal Antibody (bsm-54204R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54204R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: PC-12 cells lysates; Probed with Chromogranin A (7C7)Monoclonal Antibody (bsm-52883R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with Chromogranin A (7C7) Monoclonal Antibody (bsm-52883R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52883R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with NF-H monoclonal antibody, unconjugated (bsm-52851R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. \r\n
Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-H Monoclonal Antibody, Unconjugated(bsm-52851R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-H Monoclonal Antibody, Unconjugated(bsm-52851R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NF-H Monoclonal Antibody, Unconjugated(bsm-52851R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52851r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52820r

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

IF(ICC) staining with Cardiac Troponin I (4C2) Monoclonal Antibody (bsm-52818R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Western blot analysis of fetal heart cell lysate. Using Cardiac Troponin I\/TNNC1 (bsm-52818R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cardiac Troponin I\/TNNC1 Monoclonal Antibody, Unconjugated(bsm-52818R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining and DAB (C-0010) staining.

Catalog #1: bsm-52818r

Applications: WB, IHC-P, IHC-F, IP, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with CD99 (3C3) Monoclonal Antibody (bsm-52816R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: THP-1 cells lysates; Probed with CD99 (3C3) Monoclonal Antibody (bsm-52816R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with CD99 (3C3) Monoclonal Antibody (bsm-52816R) at 1:100 in PANC-1 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with CD99 (3C3) Monoclonal Antibody (bsm-52816R) at 1:100 in PC-3M cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52816R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: 293T lysates; Lane 2: Hela lysates; Probed with Thymidine Kinase 1 (9C1)Monoclonal Antibody (bsm-52813R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100 in NIH\/3T3 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Thymidine Kinase 1 (9C1) Monoclonal Antibody (bsm-52813R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52813r

Applications: WB, IHC-P, IP, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Alpha Actinin 2 Monoclonal Antibody, Unconjugated (bsm-52812R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52812r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293 Cells; Lane 2: MCF-7 Cells; Lane 3: Hela Cells; Probed with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:300 in LO2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Insulin Receptor Alpha (12C5) Monoclonal Antibody (bsm-52644R) at 1:300 in RH-35 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52644R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1:Jurkat Cells; Lane 2: HepG2 Cells; Probed with BRG1 (6H10) Monoclonal Antibody (bsm-52638R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with BRG1 (6H10) Monoclonal Antibody (bsm-52638R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with BRG1 (6H10) Monoclonal Antibody (bsm-52638R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with BRG1 (6H10) Monoclonal Antibody (bsm-52638R) at 1:100 in SW480 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52638R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Mouse eyeball lysate probed with Phospho-PKC zeta (Thr560) (26G8) Monoclonal Antibody (bsm-52632R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with PKC zeta (Thr560) (26G8) Monoclonal Antibody (bsm-52632R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PKC zeta (Thr560) (26G8) Monoclonal Antibody (bsm-52632R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52632R

Applications: WB, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 Cells; Lane 2: Jurkat Cells; Lane 3: Mouse Colon; Probed with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Skin tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Kidney tissue incubated with PTBP1 (7D3) Monoclonal Antibody (bsm-54208R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54208R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Placenta tissue lysates; Lane 2: Rat Placenta tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human HepG2 cell lysates probed with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Human U251 cell lysates; Lane 4: Human HUVEC cell lysates; Lane 5: Human LOVO cell lysates probed with Nuclear Matrix Protein p84 Monoclonal Antibody, Unconjugated (bsm-54202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-54202r

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Mouse Heart tissue incubated with FABP3 (4C4) Monoclonal Antibody (bsm-54200R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Rat Heart; Probed with FABP3 (4C4) Monoclonal Antibody (bsm-54200R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with FABP3 (4C4) Monoclonal Antibody (bsm-54200R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54200R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Placenta; Lane 2: Mouse Testis; Lane 3: Hela Cells; Lane 4: A431 Cells; Lane 5: MCF-7 Cells; Probed with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TPT1 (7C1) Monoclonal Antibody (bsm-54198R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54198R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Brain; Lane 2: 293T Cells; Lane 3: Rat Liver; Lane 4: Mouse Colon; Probed with Carbonic anhydrase 2 (6C1) Monoclonal Antibody (bsm-54197R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Stomach tissue incubated with Carbonic anhydrase 2 (6C1) Monoclonal Antibody (bsm-54197R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Carbonic anhydrase 2 (6C1) Monoclonal Antibody (bsm-54197R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Stomach tissue incubated with Carbonic anhydrase 2 (6C1) Monoclonal Antibody (bsm-54197R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54197R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Human Liver tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Cerebellum tissue incubated with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Hela Cells; Lane 2: Jurkat Cells; Lane 3: NIH-3T3 Cells; Lane 4: Rat Brain; Lane 5: Mouse Brain; Probed with SPTAN1 (4C8) Monoclonal Antibody (bsm-54194R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-54194R

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Brain; Lane 2: SK-Br-3 Cells; Lane 3: Rat Lung; Probed with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in A431 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated with PTGES3 (1A26) Monoclonal Antibody (bsm-54193R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54193R

Applications: WB, IHC-P, IF(ICC), IP, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with ZAP70 monoclonal antibody, unconjugated (bsm-54192R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human tonsils; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZAP70 Monoclonal Antibody, Unconjugated(bsm-54192R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human lymph nodes ;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZAP70 Monoclonal Antibody, Unconjugated(bsm-54192R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
\uff08Negative control\uff09Paraformaldehyde-fixed, paraffin embedded Human liver ;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ZAP70 Monoclonal Antibody, Unconjugated(bsm-54192R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54192r

Applications: WB, IHC-P, IHC-F, IP, IF, Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-54190r

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Human Serum; Probed with WASL (9C3) Monoclonal Antibody (bsm-54189R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with WASL (9C3) Monoclonal Antibody (bsm-54189R) at 1:100 in SL-Br-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with WASL (9C3) Monoclonal Antibody (bsm-54189R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human Spleen tissue incubated with WASL (9C3) Monoclonal Antibody (bsm-54189R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54189R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SDHB Monoclonal Antibody, Unconjugated(bsm-54180R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SDHB Monoclonal Antibody, Unconjugated(bsm-54180R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SDHB Monoclonal Antibody, Unconjugated(bsm-54180R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SDHB Monoclonal Antibody, Unconjugated(bsm-54180R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54180r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: THP-1 Cells; Probed with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SAMHD1 (16C6) Monoclonal Antibody (bsm-54174R) at 1:300 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54174R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with alpha Internexin monoclonal antibody, unconjugated (bsm-54173R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation alpha Internexin Monoclonal Antibody, Unconjugated(bsm-54173R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation alpha Internexin Monoclonal Antibody, Unconjugated(bsm-54173R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation alpha Internexin Monoclonal Antibody, Unconjugated(bsm-54173R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54173r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: HepG2 Cells; Lane 2: NIH-3T3 Cells; Lane 3: MCF-7 Cells; Lane 4: Rat Kidney; Probed with DDB1 (1C1) Monoclonal Antibody (bsm-54172R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with DDB1 (1C1) Monoclonal Antibody (bsm-54172R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with DDB1 (1C1) Monoclonal Antibody (bsm-54172R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with DDB1 (1C1) Monoclonal Antibody (bsm-54172R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54172R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: Raji Cells; Lane 3: MCF-7 Cells; Lane 4: Jurkat Cells; Probed with IKBKE (6C1) Monoclonal Antibody (bsm-54171R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with IKBKE (6C1) Monoclonal Antibody (bsm-54171R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with IKBKE (6C1) Monoclonal Antibody (bsm-54171R) at 1:100 in JAR cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with IKBKE (6C1) Monoclonal Antibody (bsm-54171R) at 1:100 in SK-Br-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54171R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with Cathepsin LVKH (9C3) Monoclonal Antibody (bsm-52919R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Lane 1: HepG2 cells lysates; Lane 2: A549 brain cells lysates; Probed with Cathepsin LVKH (9C3) Monoclonal Antibody (bsm-52919R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with Cathepsin LVKH (9C3) Monoclonal Antibody (bsm-52919R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse kidney tissue incubated with Cathepsin LVKH (9C3) Monoclonal Antibody (bsm-52919R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52919R

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-52917r

Applications: WB, ICC/IF

Reactivity: Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: 293 Cells; Probed with SHP2 (8A1F) Monoclonal Antibody (bsm-52633R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SHP2 (8A1F) Monoclonal Antibody (bsm-52633R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SHP2 (8A1F) Monoclonal Antibody (bsm-52633R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SHP2 (8A1F) Monoclonal Antibody (bsm-52633R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52633R

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse Brain lysate probed with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100 in PMVEC cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.\r\n\r\n
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with MAP1LC3A (1C7) Monoclonal Antibody (bsm-52595R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52595R

Applications: WB, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: C2C12 Cells; Lane 2: A431 Cells; Probed with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with COX1\/Cyclooxygenase 1 (4A8) Monoclonal Antibody (bsm-52569R) at 1:300 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52569R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: Mouse Placenta; Lane 3: MCF-7 Cells; Probed with H2AFY (6C8) Monoclonal Antibody (bsm-54167R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with H2AFY (6C8) Monoclonal Antibody (bsm-54167R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with H2AFY (6C8) Monoclonal Antibody (bsm-54167R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with H2AFY (6C8) Monoclonal Antibody (bsm-54167R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54167R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela Cells; Lane 2: PC-12 Cells; Lane 3: NIH-3T3 Cells; Lane 4: Rat Liver; Lane 5: A431 Cells; Lane 6: HepG2 Cells; Probed with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 in Hela cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ACTN4 (6A1) Monoclonal Antibody (bsm-54157R) at 1:100 in LOVO cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-54157R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Mouse Colon tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Flow cytometric analysis of Hela cells with CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed and paraffin-embedded Human Placenta tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Tonsil tissue incubated CBX3 (4B4) Monoclonal Antibody (bsm-54154R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54154R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with MMP3 monoclonal antibody, unconjugated (bsm-54152R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP3 Monoclonal Antibody, Unconjugated (bsm-54152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP3 Monoclonal Antibody, Unconjugated (bsm-54152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with MMP3 Monoclonal Antibody, Unconjugated (bsm-54152R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54152r

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed and paraffin-embedded Mouse Testis tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Pancreas tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Stomach Cancer tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Rat Bratin tissue incubated with Histone H2AX (9C9) Monoclonal Antibody (bsm-54151R) at 1:250, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54151R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

THP-1 (H) cells were treated with or without LPS (1 ug\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with TLR2 monoclonal antibody, unconjugated (bsm-54147R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLR2 Monoclonal Antibody, Unconjugated(bsm-54147R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLR2 Monoclonal Antibody, Unconjugated(bsm-54147R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thumus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TLR2 Monoclonal Antibody, Unconjugated(bsm-54147R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-54147r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse

Conjugate: Unconjugated