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Monoclonal IHC Antibodies



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Lane 1: HT-29 Cell lysates; probed with AURKA (4C5) Monoclonal Antibody, unconjugated (bsm-51279M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with AURKA (4C5) Monoclonal Antibody (bsm-51279M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51279M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51269M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: U-251 MG Cell lysates; probed with EPHA5 (46CT61.6.4) Monoclonal Antibody, unconjugated (bsm-51268M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human Brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with EPHA5 (46CT61.6.4) Monoclonal Antibody (bsm-51268M) at 1:50 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51268M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with FYN (2C7) Monoclonal Antibody (bsm-51263M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
293 cells probed with FYN (2C7) Monoclonal Antibody, unconjugated (bsm-51263M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Human Hela Cells probed with FYN (2C7) Monoclonal Antibody, Unconjugated (bsm-51263M) (green) at 1:25 for 60 minutes followed by a Alexa Fluor\u00ae 488 goat anti-mouse lgG at 1:400. The graph shows FYN staining compared a mouse IgG1 isotype control (blue).

Catalog #1: bsm-51263M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NFKB1 (8C3) Monoclonal Antibody (bsm-51258M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human tonsil tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NFKB1 (8C3) Monoclonal Antibody (bsm-51258M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51258M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51251M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

25 ug total protein per lane of various lysates (see on figure) probed with PIN1 monoclonal antibody, unconjugated (bsm-51230M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with PIN1 Monoclonal Antibody, Unconjugated (bsm-51230M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-51230m

Applications: IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-51225M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human testis tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: HeLa; Lane 2: HepG2; Lane 3: HIN\/3T3 cell lysate probed with TBP (8C4) Monoclonal Antibody (bsm-51220M) at 1:1000 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

Catalog #1: bsm-51220M

Applications: WB, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: A431; Lane 3: HeLa; Lane 4: HepG2; Lane 5: NIH\/3T3; Lane 6: mouse stomach; Lane 7: mouse liver lysate at 35 \u00b5g per lane. probed with bsm-51216M ENO1 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with ENO1 (7C4) Monoclonal Antibody (bsm-51216M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51216M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: WiDr; Lane 2: mouse stomach lysate at 20 \u00b5g per lane, probed with bsm-51214M CDH1 (813CT11.1.3) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Paraformaldehyde-fixed, paraffin embedded human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with CDH1 (813CT11.1.3) Monoclonal Antibody (bsm-51214M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51214M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51201M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

NCCIT cell lysate probed with bsm-51142M SOX2 (7C4) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.
Lane 1: SOX2 Protein Isolate; probed with SOX2 (7C4) Monoclonal Antibody, unconjugated (bsm-51142M) at 1:4000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:50 (37\u2103 for 1hr) in A549 cells (green). Cells were counterstained with Alexa Fluor\u00ae 555 (red) conjugated Phalloidin. Cells were fixed in 4% paraformaldehyde for 20min and permeabilized with 0.1% Triton X100\/PBS for 10min.
Paraformaldehyde-fixed and paraffin-embedded Human Lung Cancer tissue incubated with SOX2 (7C4) Monoclonal Antibody (bsm-51142M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-51142M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-2022M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Virus

Conjugate: HRP

Catalog #1: bsm-4533M-HRP

Applications: IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-4533M-Biotin

Applications: IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bsm-2011M-HRP

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bsm-2011M-Biotin

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bsm-0855m-hrp-100ul

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0855m-biotin-100ul

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-2000M-HRP

Applications: ELISA, IHC-P

Reactivity: Virus, Others

Conjugate: HRP

Catalog #1: bsm-2000M-Biotin

Applications: ELISA, IHC-P

Reactivity: Virus, Others

Conjugate: Biotin

Catalog #1: bsm-2313M-HRP

Applications: WB, IHC-P

Reactivity:

Conjugate: HRP

Catalog #1: bsm-2313M-Biotin

Applications: WB, IHC-P

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-2313M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-2279M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: HRP

Catalog #1: bsm-2279M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bsm-1625M

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-1624M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0388M-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0388M-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-1624M

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-2182M-HRP

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-2182M-Biotin

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bsm-0387m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-1622M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0356M-HRP

Applications: IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bsm-1622m

Applications: WB, ELISA, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-2141M-HRP

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-1621m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-2141M-Biotin

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-0107m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bsm-1240M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0990M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: HRP

Catalog #1: bsm-0990M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Virus

Conjugate: Biotin

Catalog #1: bsm-2079M-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Other Species

Conjugate: HRP

Catalog #1: bsm-2079M-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Other Species

Conjugate: Biotin

Catalog #1: bsm-2059M-Biotin

Applications: ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin