Podoplanin Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Podoplanin) Monoclonal Antibody, Unconjugated (bsm-34236M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Podoplanin) Monoclonal Antibody, Unconjugated (bsm-34236M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Human

Overview
Catalog #	bsm-34236m-100ul
Product Name	Podoplanin Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant mouse podoplanin protein
Clonality	Monoclonal
Clone #	5C9
Isotype	IgG2b/Kappa
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	10630
Swiss Prot	Q86YL7
Subcellular location	Cytoplasm, Cell membrane
Synonyms	PDPN_HUMAN; DPN; GP36; Aggrus; Glycoprotein 36 (Gp36); PA2.26 antigen; T1-alpha; T1A; 29kDa cytosolic podoplanin intracellular domain; PICD; PSEC0003; PSEC0025;
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:50-200
IHC-F	IHC-F1:50-200
IF(IHC-P)	IFIHC-P1:50-200