ADK Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADK Monoclonal Antibody, Unconjugated (bsm-51668M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ADK Monoclonal Antibody, Unconjugated (bsm-51668M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51668m
Product Name	ADK Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human ADK between 1-345 amino acids.
Clonality	Monoclonal
Clone #	N3W5
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	132
Swiss Prot	P55263
Subcellular location	Cytoplasm, Nucleus
Synonyms	Adenosine kinase; AK; 5033405D03Rik; AI255373; AI987814; MGC6593; 2310026J05Rik; Adenosine 5'-phosphotransferase; OTTHUMP00000019864; OTTHUMP00000019865; ADK_HUMAN; Adenosine kinase; Full=Adenosine 5'-phosphotransferase.
Background	ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.
Application Dilution
WB	=1:500-4000
IHC-P	IHC-P=1:25
IHC-F	IHC-F=1:25
IF	IF=1:25