Caveolin-2 Recombinant Antibody

$4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Caveolin-) monoclonal Antibody, unconjugated (bsm-61101R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Caveolin-) monoclonal Antibody, unconjugated (bsm-61101R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$25 ug total protein per lane of various lysates (see on figure) probed with Caveolin-2 monoclonal antibody, unconjugated (bsm-61101R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Caveolin-2 monoclonal antibody, unconjugated (bsm-61101R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)
IP

Reactivity

Human

Overview
Catalog #	bsm-61101r-TR
Product Name	Caveolin-2 Recombinant Antibody
Applications	WB, IF(ICC), IP
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Caveolin 2
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	858
Swiss Prot	P51636
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	CAV2; Caveolae protein 20 Kd; Caveolin 2; Caveolin 2 isoform a and b; Caveolin-2.
Background	May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200
IP	IP1:20-50