INPP4B Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with INPP4B monoclonal antibody, unconjugated (bsm-61303R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with INPP4B monoclonal antibody, unconjugated (bsm-61303R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with INPP4B Monoclonal Antibody, Unconjugated (bsm-61303R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)

Reactivity

Mouse

Predicted Reactivity

Human
Rat

Overview
Catalog #	bsm-61303r-TR
Product Name	INPP4B Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF(IHC-P)
Reactivity	Mouse
Predicted Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human INPP4B
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8821
Swiss Prot	O15327
Subcellular location	Cytoplasm
Synonyms	4-bisphosphate 4-phosphatase; Inositol polyphosphate 4 phosphatase type II 105kDa; Inositol polyphosphate 4-phosphatase type II; Inpp4b; MGC132014; Type II inositol 3,4 bisphosphate 4 phosphatase; Type II inositol-3.
Background	Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate. Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles.The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival
Application Dilution
WB	1:500-2000
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500