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Muscular Dystrophies and Neuromuscular Diseases



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Lane 1: Rat Heart; Probed with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Smooth Muscle tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human Heart tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse Skeletal Muscle tissue incubated with Tropomyosin 1 (2G2) Monoclonal Antibody (bsm-54406R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-54406R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Mouse Heart; Probed with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:500, overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Rat Heart tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Colon tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.
Paraformaldehyde-fixed and paraffin-embedded Human Fetal Skeletal Muscle tissue incubated with ACTN2 (7G3) Monoclonal Antibody (bsm-54465R) at 1:200, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin. The tissue was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.

Catalog #1: bsm-54465R

Applications: WB, IHC-P, IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-19924R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-19924R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-19924R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-11055r

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-11055R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-11055R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TNNI3 Polyclonal Antibody, Unconjugated (bs-10614R) at 1:500 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TNNI3 Polyclonal Antibody, Unconjugated (bs-10614R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Heart lysates; Lane 2: Rat Heart lysates probed with TNNI3 Polyclonal Antibody, Unconjugated (bs-10614R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10614R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-10614R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-10614R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-5551R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-5551R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-16635R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-23012R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-23012R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-19170R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-19170R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-19170R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-21460R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-16635R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-16635R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lamin A\/C Monoclonal Antibody, Unconjugated (bsm-34042M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lamin A\/C Monoclonal Antibody, Unconjugated (bsm-34042M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lamin A\/C Monoclonal Antibody, Unconjugated (bsm-34042M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Lamin A\/C Monoclonal Antibody, Unconjugated (bsm-34042M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-34042m

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-21455R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-21455R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Desmin) Monoclonal Antibody, Unconjugated (bsm-60266R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with Desmin monoclonal antibody, unconjugated (bsm-60266R) at 1:5000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Mouse Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Desmin Monoclonal Antibody, Unconjugated(bsm-60266R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Desmin Monoclonal Antibody, Unconjugated(bsm-60266R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-60266r-TR

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1:HeLa, 2:HEK-293, 3:4T1, 4:Raw264.7\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 100 kDa\r\nObserved MW: 110 kDa\r\n
Tissue: Human colon Section type: Formalin fixed & Paraffin -embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at room temperature Secondary ab: SP Kit(Rabbit) (sp-0023) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for bsm-60423R
Cell line: HeLa\r\nFixative: 4% Paraformaldehyde\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60423R
Lane 1: Mouse Skin tissue lysates; Lane 2: Mouse Uterus tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human U251 cell lysates probed with alpha Actinin 4 Monoclonal Antibody, Unconjugated (bsm-60423R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60423r

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Tissue: Human placenta\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60601R
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Laminin B1 monoclonal antibody, unconjugated (bsm-60601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-60601r

Applications: WB, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (Rat bladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Desmin) Monoclonal Antibody, Unconjugated (bsm-33229M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Desmin) Monoclonal Antibody, Unconjugated (bsm-33229M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Desmin) Monoclonal Antibody, Unconjugated (bsm-33229M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded (Rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Desmin) Monoclonal Antibody, Unconjugated (bsm-33229M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33229m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Caveolin-1) Monoclonal Antibody, Unconjugated (bsm-60809R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Caveolin-1) Monoclonal Antibody, Unconjugated (bsm-60809R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Caveolin-1) Monoclonal Antibody, Unconjugated (bsm-60809R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60809r

Applications: IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-12637R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-12600R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-10902R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-20702R-HRP

Applications: IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-20702R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-20702R

Applications: IHC-P, IHC-F, IF(ICC), IF

Reactivity: Rat

Conjugate: Unconjugated