Integrin Alpha V + Beta 1 Polyclonal Antibody

Lane 1: Human A549 cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human SH-SY5Y cell lysates; Lane 5: Human U-2OS cell lysates; Lane 6: Human MCF-7 cell lysates; Lane 7: Human Jurkat cell lysates probed with Integrin Alpha V + Beta1 Polyclonal Antibody, Unconjugated (bs-2016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
FCM
IHC-P
IF(ICC)

Reactivity

Human
Rat

Predicted Reactivity

Mouse
Dog
Cow
Sheep
Pig
Horse

Overview
Catalog #	bs-2016R
Product Name	Integrin Alpha V + Beta 1 Polyclonal Antibody
Applications	WB, ELISA, FCM, IHC-P, IF(ICC)
Reactivity	Human, Rat
Predicted Reactivity	Mouse, Dog, Cow, Sheep, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Mixed KLH conjugated syntheticpeptides for human Integrin Alpha V and human Integrin Beta1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3685, 3688
Subcellular location	Cytoplasm, Secreted
Synonyms	Integrin alpha-V: Integrin alpha-V, Vitronectin receptor subunit alpha, CD51 Integrin beta-1: Fibronectin receptor subunit beta, Glycoprotein IIa, GPIIA, VLA-4 subunit beta, CD29
Background	The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200