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NEURO10 Applicable Antibodies



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Formalin-fixed and paraffin embedded: rat sciatic nerve labeled with Anti-AGER\/RAGE Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
NIH\/3T3 cells probed with Rabbit Anti-RAGE Polyclonal Antibody (bs-0177R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.\\n
Raji Cells lysates probed with RAGE Polyclonal Antibody, unconjugated (bs-0177R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAGE Polyclonal Antibody(bs-0177R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0177R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated (bs-1099R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded mouse liver tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated (bs-1099R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-Calpain 1 Polyclonal Antibody, Unconjugated (bs-1099R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human MCF7 cells probed with Calpain 1 Polyclonal Antibody, unconjugated (bs-1099R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.\r\n

Catalog #1: bs-1099R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1099R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0177R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin