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NEURO10 Applicable Antibodies



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Catalog #1: bsm-0107m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Lane 1: L6; Lane 2: NIH\/3T3; Lane 3: T47D; Lane 4: A2058; Lane 5: HepG2; Lane 6: K562; Lane 7: HeLa Cell lysates; probed with Hsp90 (3C9) Monoclonal Antibody, unconjugated (bsm-51215M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Placenta tissue lysates; Lane 4: Rat Cerebrum tissue lysates ; Lane 5: Rat Placenta tissue lysates ; Lane 6: Human U251 cell lysates ; Lane 7: Human Molt-4 cell lysates ; Lane 8: Human HL60 cell lysates ; Lane 9: Human A549 cell lysates probed with HSP90 Monoclonal Antibody, Unconjugated (bsm-51215M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-51215m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: 293T; Lane 2: A431 probed with bsm-51265M PINK1 (38CT20.8.5) Monoclonal Antibody at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human stomach tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PINK1 (38CT20.8.5) Monoclonal Antibody (bsm-51265M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bsm-51265m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

HeLa Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 Cell lysates; probed with PPT1 (10G4) Monoclonal Antibody, unconjugated (bsm-51262M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human cerebellum tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PPT1 (10G4) Monoclonal Antibody (bsm-51262M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
HepG2 cells probed with using PPT1 (10G4) Monoclonal Antibody (green) unconjugated bsm-51262M at 1:25 for 60 minutes at 37\u00baC followed by secondary antibody at 1:200 compared to an isotype control of mouse IgG (blue).

Catalog #1: bsm-51262M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse lymphoma tissue labeled with Anti-TNF-alpha Polyclonal Antibody, FITC conjugated (bsm-0387M-FITC) at 1:200 followed by conjugation to the secondary antibody
Formalin-fixed and paraffin embedded:human colon labeled with Anti-TNF-Alpha Polyclonal Antibody, Unconjugated (bsm-0387M), followed by conjugation to the secondary antibody and DAB staining
4% Paraformaldehyde-fixed THP-1(LPS treated (100 ng\/ml, 16 h) and Brefeldin A treated (5 \u00b5g\/ml, 4 h)) (H) cell; Antibody incubation with (TNF alpha) monoclonal Antibody, unconjugated (bsm-0387M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Blank control:THP-1. Primary Antibody (green line): Mouse Anti-TNF alpha antibody (bsm-0387M) Dilution: 1ug\/Test; Secondary Antibody : Goat anti-mouse IgG-FITC Dilution: 0.5ug\/Test. Protocol The cells were treated with TPA (80 nM, overnight) and then treated with LPS (1 ug\/mL, 18 hr\/6 hr) and Brefeldin A (300 ng\/mL, last 3 hr of stimulation) .The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Catalog #1: bsm-0387m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0107m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bsm-0387m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0387m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0107m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bsm-0107m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bsm-0387m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bsm-0387m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0387m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-0387m-pe-cy7-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy7

Catalog #1: bsm-0387m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5.5

Catalog #1: bsm-0387m-pe-cy5-100ul

Applications: WB

Reactivity: Human

Conjugate: PE-Cy5

Catalog #1: bsm-0387m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-0387m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-0387m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-0387m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0387m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0387m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0387m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0387m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0387m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0387m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0387m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0387m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-0107m-rbitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: RBITC

Catalog #1: bsm-0107m-percp-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-0107m-percp-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: PerCP

Catalog #1: bsm-0107m-pe-cy7-100ul

Applications: WB

Reactivity: Rat

Conjugate: PE-Cy7

Catalog #1: bsm-0107m-pe-Cy5.5-100ul

Applications: WB

Reactivity: Rat

Conjugate: PE-Cy5.5

Catalog #1: bsm-0107m-pe-cy5-100ul

Applications: WB

Reactivity: Rat

Conjugate: PE-Cy5

Catalog #1: bsm-0107m-apc-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: APC-Cy7

Catalog #1: bsm-0107m-apc-Cy5.5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: APC-Cy5.5

Catalog #1: bsm-0107m-apc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: APC

Catalog #1: bsm-0107m-bf750-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-750

Catalog #1: bsm-0107m-bf680-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-680

Catalog #1: bsm-0107m-bf647-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-647

Catalog #1: bsm-0107m-bf594-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-594

Catalog #1: bsm-0107m-bf555-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-555

Catalog #1: bsm-0107m-bf488-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-488

Catalog #1: bsm-0107m-bf405-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-405

Catalog #1: bsm-0107m-bf350-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: AbBy Fluor-350

Catalog #1: bsm-0107m-pe-100ul

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: PE

Catalog #1: bsm-60415m

Applications: WB, ICC/IF

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-51545m

Applications: WB, IHC-P, IHC-F, IF

Reactivity:

Conjugate: Unconjugated