Neurokinin A Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Anti-NKA\/Neurokinin A Polyclonal Antibody, Unconjugated (bs-0069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NKA\/Neurokinin A Polyclonal Antibody, Unconjugated (bs-0069R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin-embedded rat brain labeled with Anti-NKA\/Neurokinin A Polyclonal Antibody, Unconjugated(bs-0069R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.$

Formalin-fixed and paraffin-embedded rat brain labeled with Anti-NKA\/Neurokinin A Polyclonal Antibody, Unconjugated(bs-0069R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

$Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human U-2 OS cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human u251 cell lysates probed with Neurokinin A Polyclonal Antibody, Unconjugated (bs-0069R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Stomach tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Human U-2 OS cell lysates; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human u251 cell lysates probed with Neurokinin A Polyclonal Antibody, Unconjugated (bs-0069R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Horse
Chicken
Rabbit

Overview
Catalog #	bs-0069R
Product Name	Neurokinin A Polyclonal Antibody
Applications	ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NKA
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6863
Swiss Prot	P20366
Subcellular location	Secreted
Synonyms	NK2; NPK; NKNA; TAC2; Hs.2563; Protachykinin-1; PPT; TAC1; NKA
Background	Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.
Application Dilution
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200