NRSF Polyclonal Antibody

$Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF\/REST Polyclonal Antibody,Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded human gastric tissue labeled with Anti NRSF\/REST Polyclonal Antibody,Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

$Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.$

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Raji lysates probed with NRSF Polyclonal Antibody, Unconjugated (bs-2590R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Lane 1: Hela cell lysates; Lane 2: Hela KO NRSF cell lysates probed with NRSF Polyclonal Antibody, Unconjugated (bs-2590R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: human hela cell lysates; Lane 2: bs-2590R IP in human hela cell lysates; Lane 3: native rabbit IgG IP in human hela cell lysates probed with NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:150 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: human hela cell lysates; Lane 2: bs-2590R IP in human hela cell lysates; Lane 3: native rabbit IgG IP in human hela cell lysates probed with NRSF\/REST Polyclonal Antibody, Unconjugated (bs-2590R) at 1:150 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Cow
Pig
Horse
Chicken
Rabbit

Overview
Catalog #	bs-2590R
Product Name	NRSF Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Specificity	KO-Validated
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NRSF
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5978
Swiss Prot	Q13127
Subcellular location	Nucleus
Synonyms	XBR; NRSF; RE1-silencing transcription factor; Neural-restrictive silencer factor; X2 box repressor; REST
Background	Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200