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Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bs-0061R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: HRP

Catalog #1: bs-0061R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Biotin

Catalog #1: bs-0061R-Cy3

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy3

Catalog #1: bs-0061R-Cy5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5

Catalog #1: bs-0061R-FITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: FITC

Catalog #1: bs-0061R-Cy7

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy7

Catalog #1: bs-0061R-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Cy5.5

Catalog #1: bs-0061R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE

Catalog #1: bs-3806R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: Cy5.5

Catalog #1: bs-3806R-PE

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: PE

Catalog #1: bs-3806R-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-350

Catalog #1: bs-3806R-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-405

Catalog #1: bs-3806R-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-488

Catalog #1: bs-3806R-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-555

Catalog #1: bs-3806R-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-594

Catalog #1: bs-3806R-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-647

Catalog #1: bs-3806R-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-680

Catalog #1: bs-3806R-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: AbBy Fluor-750

Catalog #1: bs-3806R-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: APC

Catalog #1: bs-3806R-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: APC-Cy5.5

Catalog #1: bs-3806R-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: APC-Cy7

Catalog #1: bs-3806R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Cow

Conjugate: PE-Cy5

Catalog #1: bs-3806R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Cow

Conjugate: PE-Cy5.5

Catalog #1: bs-3806R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Cow

Conjugate: PE-Cy7

Catalog #1: bs-3806R-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: PerCP

Catalog #1: bs-3806R-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: PerCP-Cy5.5

Catalog #1: bs-3806R-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Cow

Conjugate: RBITC

Catalog #1: bs-0061R-BF350

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-350

Catalog #1: bs-0061R-BF405

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-405

Catalog #1: bs-0061R-BF488

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-488

Catalog #1: bs-0061R-BF555

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-555

Catalog #1: bs-0061R-BF594

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-594

Catalog #1: bs-0061R-BF647

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-647

Catalog #1: bs-0061R-BF680

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-680

Catalog #1: bs-0061R-BF750

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: AbBy Fluor-750

Catalog #1: bs-0061R-APC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC

Catalog #1: bs-0061R-APC-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy5.5

Catalog #1: bs-0061R-APC-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: APC-Cy7

Catalog #1: bs-0061R-PE-Cy5

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5

Catalog #1: bs-0061R-PE-Cy5.5

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy5.5

Catalog #1: bs-0061R-PE-Cy7

Applications: WB

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PE-Cy7

Catalog #1: bs-0061R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP

Catalog #1: bs-0061R-PerCP-Cy5.5

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0061R-RBITC

Applications: WB, IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: RBITC