Receive 30% off all Monoclonal IHC antibodies with code "MONO-IHC-30"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Nuclear Antibodies



×

Collection Menu
Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody (bs-0465R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated (bs-0465R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated (bs-0465R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).

Catalog #1: bs-0465R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Mouse lung lysates probed with Rabbit Anti-NFkB p65 (Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse spleen lysates probed with Rabbit Anti-NFKB p65(Ser536) Polyclonal Antibody, Unconjugated (bs-0982R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C
HL-60 cells probed with NFKB p65(Ser536) Polyclonal Antibody, unconjugated (bs-0982R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0982R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65(Ser276) Polyclonal Antibody, Unconjugated (bs-3543R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65(Ser276) Polyclonal Antibody, Unconjugated (bs-3543R) at 1:400 overnight at 4\u00b0C, DAB staining.
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Phospho-NFKB p65 (Ser276) Antibody(bs-3543R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65(Ser276) Polyclonal Antibody, Unconjugated (bs-3543R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3543R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti PARP (N-Terminus) Polyclonal Antibody, Unconjugated(bs-2138R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI
Lane 1: Rat Brain; Probed with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:1000 in 4˚C.
Human HeLa lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Human Raji lysates probed with Rabbit Anti-PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-2138R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat thymus tissue labeled Anti-PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
293T lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human Hela cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Rat Large intestine lysates probed with PCNA Polyclonal Antibody, Unconjugated (bs-0754R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0754R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled Anti-HMGB1 Polyclonal Antibody, Unconjugated (bs-0664R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Macrophages probed with Rabbit Anti-HMGB1 Polyclonal Antibody, Unconjugated (bs-0664R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Human HepG2 cell lysates probed with Rabbit Anti-HMGB1 Polyclonal Antibody, PE-Cy5 conjugated (bs-0664R-PE-Cy5) (green) at 1:20 for 30 minutes followed compared to unstained cells (blue) and PE-Cy5 isotype control bs-0295P-PE-Cy5(orange).
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HMGB1 Polyclonal Antibody(bs-0664R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0664R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NFkB Inducing Kinase NIK Polyclonal Antibody, Unconjugated (bs-0074R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: mouse kidney, lysates Lane 2: mouse brain probed with Rabbit Anti-NFkB Inducing Kinase NIK Polyclonal Antibody, Unconjugated (bs-0074R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.

Catalog #1: bs-0074R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti-NFKB Polyclonal Antibody, Unconjugated (bs-10037R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
MCF-7 cells were stained with NFKB Polyclonal Antibody, Unconjugated(bs-10037R) at 1:500 in PBS and incubated for two hours at 37\u00b0C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

Catalog #1: bs-10037R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of HeLa lysate probed with \r\nPCNA (1G3) Monoclonal Antibody (bsm-52347R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA (1G3) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-52347r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0803R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Raji cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-20160R) at 1:200 dilution and 4ûC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37ûC.

Catalog #1: bs-20160R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human gastric tissue labeled with Rabbit Anti-NFkB p105 \/ p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Mouse lung lysates probed with Rabbit Anti-NFkB p105\/p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFkB p105 \/ p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFkB p105 \/ p50 Polyclonal Antibody, Unconjugated (bs-1194R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1194R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Spleen lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.
Hela lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 lysates probed with NFKBIA\/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1287R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1839R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PCNA (3G1) Monoclonal Antibody(bs-R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with PCNA(Nuclear Loading Control) Monoclonal Antibody, Unconjugated(bsm-33035M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Catalog #1: bsm-33035m

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-11678R

Applications: WB, ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: Human MOLT-4 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human RAW264.7 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-5663R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Pancreas lysates; Lane 2: Human HL-60 cell lysates probed with NFKB p65 Polyclonal Antibody, Unconjugated (bs-5663R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-5663R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: NIH\/3T3 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: A431 cell lysates probed with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PCNA(1C11) Monoclonal Antibody, Unconjugated (bsm-2006M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-2006M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Lane 1: Rela-KO cell lysates; Lane 2: Hela cell lysates probed with NFKB p65 (7G6) Monoclonal Antibody, Unconjugated (bsm-33117M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
25 ug total protein per lane of various lysates (see on figure) probed with NFKB p65 monoclonal antibody, unconjugated (bsm-33117M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, unconjugated (bsm-33117M) 1:50, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-40296G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated(bsm-33117M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33117m

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0465R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NFKBIA(Ser32\/36) Polyclonal Antibody, Unconjugated (bs-2513R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Siha cell lysates; Lane 4: Human U251 cell lysates probed with NFKBIA Polyclonal Antibody, Unconjugated (bs-2513R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-IKB alpha (Ser32 + Ser36)) polyclonal Antibody, Unconjugated (bs-2513R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-2513R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Molt-4 cell lysates; Lane 2: Siha cell lysates; Lane 3: A549 cell lysates probed with HP1 alpha Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (bsm-33377M) at 1:100 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33377m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-13495R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Tissue\/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37\u00b0C for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, Unconjugated (bsm-33059M) 1:100, 90 minutes at 37\u00b0C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
25 ug total protein per lane of various lysates (see on figure) probed with NFKB p65 monoclonal antibody, unconjugated (bsm-33059M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of bsm-33059M) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with NFKB p65 Monoclonal Antibody, Unconjugated (ascites of bsm-33059M) at 1:1500 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33059m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human nasopharyngeal carcinoma with Anti-BRCA1 Polyclonal Antibody, Unconjugated (bs-0802R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human Nasopharyngeal Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with BRCA1 Polyclonal Antibody, Unconjugated (bs-0802R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Neurilemmoma Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with BRCA1 Polyclonal Antibody, Unconjugated (bs-0802R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with BRCA1 Polyclonal Antibody, Unconjugated (bs-0802R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0802R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0664R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0664R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10037R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-11678R-Biotin

Applications: WB, ELISA

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-13371R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

HL-60 cells were fixed with 4% PFA for 10min at room temperature\uff0c0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRCA1 (Ser1524) Polyclonal Antibody at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Control cells (dark blue), secondary only (light blue), Isotype control (orange) and BRCA1 (Ser1524) (green).
HL-60 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with \r\nBRCA1 (Ser1524) Polyclonal Antibody(bs-3014R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-3014R

Applications: ELISA, FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3485R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-3543R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NUP160 Polyclonal Antibody, Unconjugated (bs-7843R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NUP160 Polyclonal Antibody, Unconjugated (bs-7843R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-7843R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-AGFG2 Polyclonal Antibody, Unconjugated (bs-8073R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary for 90 minutes and DAB staining.
PC-3 lysates probed with AGFG2 Polyclonal Antibody, Unconjugated (bs-8073R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-8073R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9323R-Biotin

Applications: ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Others

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HMGB1 Monoclonal Antibody, Unconjugated(bsm-52250R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HMGB1 Monoclonal Antibody, Unconjugated(bsm-52250R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HMGB1 Monoclonal Antibody, Unconjugated(bsm-52250R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with HMGB1 Monoclonal Antibody, Unconjugated(bsm-52250R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-52250r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-41239R-HRP

Applications: WB

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0465R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: APC

Catalog #1: bs-20160R-PerCP

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PerCP

Catalog #1: bs-23303R-BF488

Applications: FCM, IF

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bs-0465R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0074R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-0074R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0074R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0802R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-11678R-FITC

Applications: WB, FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-1287R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC