NUP54 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NUP54 Monoclonal Antibody, Unconjugated (bsm-51465M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with NUP54 Monoclonal Antibody, Unconjugated (bsm-51465M) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51465m
Product Name	NUP54 Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human NUP54.
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	53371
Swiss Prot	Q7Z3B4
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	54 kDa nucleoporin; MGC13407; Nucleoporin p54; NUP54_HUMAN.
Background	Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane
Application Dilution
IHC-P	=1:400-800
IHC-F	IHC-F=1:400-800
IF	IF=1:100-500