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Lane 1: HeLa Cell lysates; probed with FGFR1 (4C3) Monoclonal Antibody, unconjugated (bsm-51288M) at 1:2000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded human brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with FGFR1 (4C3) Monoclonal Antibody (bsm-51288M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells; antibody incubation with FGFR1 (4C3) Monoclonal Antibody, unconjugated (bsm-51288M) at 1:25, followed by a conjugated secondary and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (FGFR1) Monoclonal Antibody, Unconjugated (bsm-51288M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-51288M

Applications: WB, IHC-P, IF

Reactivity: Human

Conjugate: Unconjugated