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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:500 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (p53) Monoclonal Antibody, Unconjugated (bsm-60806R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
The HT-29 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-p53 antibody (bsm-60806R),1\u03bcg\/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.

Catalog #1: bsm-60806r-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-60806r-bf350-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bsm-60806r-bf405-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bsm-60806r-bf488-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bsm-60806r-bf555-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bsm-60806r-bf594-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bsm-60806r-bf647-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bsm-60806r-bf680-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bsm-60806r-bf750-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bsm-60806r-apc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bsm-60806r-apc-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bsm-60806r-apc-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bsm-60806r-cy3-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-60806r-cy5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-60806r-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-60806r-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-60806r-fitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-60806r-pbs-only-100ul

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PBS Only

Catalog #1: bsm-60806r-pe-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-60806r-percp-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bsm-60806r-percp-Cy5.5-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bsm-60806r-percp-cy7-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy7

Catalog #1: bsm-60806r-rbitc-100ul

Applications: WB, FCM, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Catalog #1: bs-3706R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-3706R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-350

Catalog #1: bs-3706R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-405

Catalog #1: bs-3706R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-488

Catalog #1: bs-3706R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-555

Catalog #1: bs-3706R-BF594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-594

Catalog #1: bs-3706R-BF647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-647

Catalog #1: bs-3706R-BF680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-680

Catalog #1: bs-3706R-BF750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: AbBy Fluor-750

Catalog #1: bs-3706R-APC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC

Catalog #1: bs-3706R-APC-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy5.5

Catalog #1: bs-3706R-APC-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: APC-Cy7

Catalog #1: bs-3706R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-3706R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-3706R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bs-3706R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-3706R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-3706R-PerCP

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP

Catalog #1: bs-3706R-PerCP-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PerCP-Cy5.5

Catalog #1: bs-3706R-RBITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: RBITC

Lane 1: Human A431 cell lysates; Lane 2: Human A549 cell lysates; Lane 3: Human MCF-7 cell lysates probed with P53 Polyclonal Antibody, Unconjugated (bs-3707R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-P53 (Ser46)) polyclonal Antibody, Unconjugated (bs-3707R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-3707R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-3707R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-350

Catalog #1: bs-3707R-BF405

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-405

Catalog #1: bs-3707R-BF488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-488

Catalog #1: bs-3707R-BF555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: AbBy Fluor-555