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Primary Antibodies



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Catalog #1: bs-8869R-FITC

Applications: FCM

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-9017R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUL1 Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUL1 Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUL1 Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295g-cy3) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MUL1 Polyclonal Antibody, Unconjugated (bs-9291R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295g-cy3) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-9291R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9291R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-9291R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-9291R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

A549 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MUL1 Polyclonal Antibody, FITC Conjugated (bs-9291R-FITC) at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
A431 cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MUL1 Polyclonal Antibody, FITC Conjugated (bs-9291R-FIT) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
A431 cells were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with 20% PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MUL1 Polyclonal Antibody, FITC Conjugated\uff08bs-9291R-FITC\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange)

Catalog #1: bs-9291R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0756R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others, Monkey

Conjugate: Cy5

Hela cells fixed with 4% paraformaldehyde; permeabilize with ice cold methanol; blocking with 1 X PBS containing 5% BSA; probed with 5 Methyl Cytosine Polyclonal Antibody, Unconjugated (bs-9450R) (green) at 1:50 for 30 minutes followed by a PE conjugated secondary antibody compared to isotype control (orange).
Raji Cells fixed with 4% paraformaldehyde; permeabilize with ice cold methanol; blocking with 1 X PBS containing 5% BSA; probed with 5 Methyl Cytosine Polyclonal Antibody, Unconjugated (bs- 9450R) (green) at 1:33 for 30 minutes followed by a PE conjugated secondary antibody compared to isotype control (orange).

Catalog #1: bs-9450R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Unconjugated

Catalog #1: bs-9450R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy5

Catalog #1: bs-9450R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy3

Catalog #1: bs-9450R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Others

Conjugate: Cy7

Catalog #1: bs-9450R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Others

Conjugate: FITC

Catalog #1: bs-9450R-PE

Applications: WB, FCM

Reactivity: Human, Others

Conjugate: PE

Catalog #1: bs-9519R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9519R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-9519R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-9519R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-9519R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Lane 1: HepG2 Cell lysates; Lane 2: K562 Cell lysates; Lane 3: NCI-H460 Cell lysates; probed with ALDH1A1 (2C2) Monoclonal Antibody, unconjugated (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: NCI-H460 Cells; 15 ug of protein lysate loaded; Probed with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human Colon Cancer tissue incubated with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
IF(ICC) staining with ALDH1A1 (2C2) Monoclonal Antibody (bsm-51032M) at 1:25 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cytoplasmic actin is detected with Dylight\u00ae 554 Phalloidin (red). Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X100\/PBS.

Catalog #1: bsm-51032M

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-51300M

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Lane 1: A431 Cell lysates; Lane 2: Mouse colon lysates; Lane 3: Mouse kidney lysates; probed with Cytokeratin 18 (1F11) Monoclonal Antibody (bsm-52058R) at 1:2000 overnight at 4˚C. Followed by a conjugated secondary antibody.
HepG2 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Cytokeratin 18 (1F11) Monoclonal Antibody (bsm-52058R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded Human breast carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cytokeratin 18 (1F11) Monoclonal Antibody (bsm-52058R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
A431 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cytokeratin 18 (1F11) Monoclonal Antibody(bsm-52058R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-52058R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Lane 1: NIH\/3T3 treated with PDGF; Lane 2: NIH\/3T3 untreatedlysates probed with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody. The bottom panel is antibody staining using an unphosphorylated AKT1 antibody.
3T3 cells were stained with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded mouse lung section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Akt1(Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
MCF7 cells (black) were fixed with 4% PFA for 10min at room temperature, permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Akt1 (Ser473) (12A1) Monoclonal Antibody (bsm-52130R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bsm-52130R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: A549 Cell lysates; probed with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.
SW480 cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS and stained with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:200 and incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.
Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Smad3 (3D1) Monoclonal Antibody (bsm-52224R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bsm-52224R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Line 1: Hela; Line 2: HepG2; Line 3: MG-63; Probed with CDT1 (2H5) Monoclonal Antibody (bsm-54038R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with Alkaline Phosphatase (1G9 ) Monoclonal Antibody (bsm-52252R) at a 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Alkaline Phosphatase (1G9) Monoclonal Antibody (bsm-52252R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52252R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: SW480; Lane 3: HCT116; Lane 4: PC12 lysate probed with ERK1\/2 (3A12) Monoclonal Antibody (bsm-52259R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with ERK1\/2 (3A12) Monoclonal Antibody (bsm-52259R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Flow cytometric analysis of SH-SY-5Y cells with ERK1\/2 antibody at 1\/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
IF(ICC) staining with ERK1\/2 (3A12) Monoclonal Antibody (bsm-52259R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52259r-100ul

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

Lane 1: MCF-7; Lane 2: Jurkat; Lane 3: Hela; Lane 4: NIH\/3T3; Probed with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Hela cells with Filamin A antibody at 1\/50 dilution (blue) compared with an unlabelled control or cells without incubation with primary antibody (red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in AGS cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Filamin A (2G3) Monoclonal Antibody (bsm-52260R) at 1:100 in HUVEC cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52260R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: Jurkat; Lane 3: 293 lysate probed with Caspase-3 (5A3) Monoclonal Antibody (bsm-52289R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with Caspase 3 (5A3) Monoclonal Antibody (bsm-52289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with Caspase 3 (5A3) Monoclonal Antibody (bsm-52289R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52289R

Catalog #1: bsm-52289r-100ul

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Line 1: MCF-7; Line 2: SK-BR-3 lysate probed with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of SH-SY-5Y cells with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
IF(ICC) staining with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:50 in N2A cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with p16 ARC (8D8) Monoclonal Antibody (bsm-52302R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52302R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Jurkat cells lysates probed with pro Caspase 3 (12B1) Monoclonal Antibody (bsm-52313R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Jpro Caspase 3 (12B1) Monoclonal Antibody (bsm-52313R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with pro Caspase 3 (12B1) Monoclonal Antibody (bsm-52313R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with pro Caspase 3 (12B1) Monoclonal Antibody (bsm-52313R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52313R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: Jurkat; Lane 3: K562 lysate probed with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of Jurkat cells with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with ERK1 (6B4) Monoclonal Antibody (bsm-52329R) at 1:300 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52329R

Applications: WB, FCM, IHC-P, IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Lane 1: SHG-44 cell lysates; Lane 2: A172 cell lysates; Lane 3: Mouse brain lysates probed with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52368r-100ul

Applications: WB, FCM, IHC-P, IHC

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated

MCF-7 cell lysates probed with LDL Receptor Polyclonal Antibody, Unconjugated (bsm-52372R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Flow cytometric analysis of HeLa lysate probed with LDL Receptor (1A7) Monoclonal Antibody (bsm-52372R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with LDL Receptor (1A7) Monoclonal Antibody (bsm-52372R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with LDL Receptor (1A7) Monoclonal Antibody (bsm-52372R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52372R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human

Conjugate: Unconjugated

Hela cell lysates probed with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Flow cytometric analysis of HeLa lysate probed with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with SUMO-1 (7H5) Monoclonal Antibody (bsm-52376R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with SUMO-1 (7H5) Monoclonal Antibody (bsm-52377R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52377R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cell lysates; Lane 2: NIH\/3T3 cell lysates; Lane 3: Mouse brain lysates probed with N Cadherin (5G1) Monoclonal Antibody (bsm-52389R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Flow cytometric analysis of HeLa cells with N Cadherin (5G1) Monoclonal Antibody (bsm-52389R) at 1:50 dilution (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with an Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes. Compared with an unlabeled control (cells without incubation with primary antibody; black).\r\n
IF(ICC) staining with N Cadherin (5G1) Monoclonal Antibody (bsm-52389R) at 1:100 in F9 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with N Cadherin (5G1) Monoclonal Antibody (bsm-52389R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52389r-100ul

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: K562 cell; Lane 2: 293 cell; Probed with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at a 1:50 dilution (blue) compared with an unlabeled control (cells without incubation with primary antibody; red).
IF(ICC) staining with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PKC alpha (2A1) Monoclonal Antibody (bsm-52410R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52410R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HepG2 cells with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; blue).
Mouse heart lysate probed with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Ubiquitin (7C6) Monoclonal Antibody (bsm-52447R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52447R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: Hela cells; Lane 2: 293 cells; Lane 3: MCF-7 cells; Lane 4: HepG2 cells; Probed with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with Hexokinase 1 (7B9) Monoclonal Antibody (bsm-52452R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52452R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Lane 1: PC12 cells; Lane 2: Jurkat cells; Probed with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100 in PC-12 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse lung tissue incubated with beta Arrestin 1 (3A11) Monoclonal Antibody (bsm-52459R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52459R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Chk1 (1A1) Monoclonal Antibody (bsm-52481R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with Chk1 (1A1) Monoclonal Antibody (bsm-52481R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Mouse liver tissue incubated with Chk1 (1A1) Monoclonal Antibody (bsm-52481R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52481R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of K562 cells with Wilms Tumor Protein (9G6) Monoclonal Antibody (bsm-52515R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Human kidney lysate probed with Wilms Tumor Protein (9G6) Monoclonal Antibody (bsm-52515R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Wilms Tumor Protein (9G6) Monoclonal Antibody (bsm-52515R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52515R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of sh-sy5y cells with Hes1 (3F8) Monoclonal Antibody (bsm-52568R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: MCF-7 Cells; Lane 2: SH-SY-5Y Cells; Probed with Hes1 (3F8) Monoclonal Antibody (bsm-52568R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with Hes1 (3F8) Monoclonal Antibody (bsm-52568R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52568R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with IKK alpha (2C2) Monoclonal Antibody (bsm-52577R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Jurkat cell lysate probed with IKK alpha (2C2) Monoclonal Antibody (bsm-52577R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed and paraffin-embedded Mouse skin tissue incubated with IKK alpha (2C2) Monoclonal Antibody (bsm-52577R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52577R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of Hela cells with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
K562 cells Probed with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Cyclin H (5G11) Monoclonal Antibody (bsm-52637R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52637R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse

Conjugate: Unconjugated

Flow cytometric analysis of HeLa cells with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
A549 cell lysate probed with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Hsp27 (7C4) Monoclonal Antibody (bsm-52757R) at 1:300 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52757R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of 293 cells with Mitofusin 1 (7C2) Monoclonal Antibody (bsm-52788R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: K562 cells lysates; Probed with Mitofusin 1 (7C2) Monoclonal Antibody (bsm-52788R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52788R

Applications: WB, FCM

Reactivity: Human

Conjugate: Unconjugated

Flow cytometric analysis of NIH\/3T3 cells with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: HUVEC cells lysates; Probed with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:300 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Src (8C4) Monoclonal Antibody (bsm-52790R) at 1:300 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52790R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Flow cytometric analysis of NIH\/3T3 cells with Glucocorticoid Receptor (1C1) Monoclonal Antibody (bsm-52797R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Lane 1: Zebra fish lysates; Lane 2: A549 lysates; Line 3: HepG2 lysates; Probed with Glucocorticoid Receptor (1C1) Monoclonal Antibody (bsm-52797R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bsm-52797R

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Zebrafish

Conjugate: Unconjugated