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Catalog #1: bs-8687R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-8687R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Mouse heart lysate probed with Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.\\n
Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Human Raji cells probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
Lane 1: Mouse Ovary; Probed with CD34 Polyclonal Antibody, Unconjugated (bs-8996R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-8996R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-8996R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy5

Catalog #1: bs-8996R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy3

Catalog #1: bs-8996R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: FITC

Catalog #1: bs-8996R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Cy7

Catalog #1: bs-8996R-PE

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: PE

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CECR1 Polyclonal Antibody, Unconjugated (bs-9005R) at 1:400 overnight at 4\u00b0C, followed by a secondary antibody for 90 minutes and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CECR1 Polyclonal Antibody, Unconjugated (bs-9005R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bs-9005R

Applications: FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9051R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9051R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-9051R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy5

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TRPM4 Polyclonal Antibody, FITC Conjugate (bs-9051R-FITC) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TRPM4 Polyclonal Antibody(bs-9051R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-9051R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-9051R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-9051R-PE

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: PE

Paraformaldehyde-fixed, paraffin embedded Rat colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MTM1 Polyclonal Antibody, Unconjugated (bs-9178R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MTM1 Polyclonal Antibody, Unconjugated (bs-9178R at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cervical carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MTM1 Polyclonal Antibody, Unconjugated (bs-9178R ) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Colon; Probed with MTM1 Polyclonal Antibody, unconjugated (bs-9178R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-9178R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9178R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-9178R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-9178R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-9178R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-9291R-PE

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: PE

Lane 1: mouse erebellum lysates; Lane 2: mouse hippocampus lysates probed with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat cerebral cortex lysates probed with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ANKRD33B Polyclonal Antibody, Unconjugated (bs-9397R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-9397R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9423R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9448R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9448R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-9448R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-9448R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-9448R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-9448R-PE

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Mouse splenocytes probed with Rabbit Anti-SIRT5 Polyclonal Antibody, Unconjugated (bs-9456R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Image provided by One World Lab validation program. PC3 cells probed with SIRT5 Polyclonal Antibody (bs-9456R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-9456R

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9456R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: Cy5

Catalog #1: bs-9456R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: Cy3

Catalog #1: bs-9456R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: Cy7

Catalog #1: bs-9456R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F)

Reactivity: Mouse

Conjugate: FITC

Catalog #1: bs-9552r

Applications: FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9552R-PE

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-9552R-Cy3

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-9552R-Cy7

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-9552R-FITC

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-9552R-Cy5

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-9553R-Cy3

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-9553R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9553R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-9553R-Cy7

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-9553R-FITC

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-DENND2D Polyclonal Antibody, Unconjugated (bs-9668R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with DENND2D Polyclonal Antibody, Unconjugated (bs-9668R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Pancreas lysates probed with DENND2D Polyclonal Antibody, Unconjugated (bs-9668R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9668R

Applications: WB, FCM, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-9668R-Cy5

Applications: WB, FCM, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5