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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-0289R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0289R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0289R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-0290R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HL-60 cells probed with Insulin Receptor Beta Antibody, unconjugated (bs-0090R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
293T lysates probed with Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-0290R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 lysates probed with Insulin Receptor Beta Polyclonal Antibody, Unconjugated (bs-0290R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0290R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0290R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0290R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0290R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0290R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0292R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Cow

Conjugate: Cy5

Antigen: bs-0292P, BSA, 1ug\/100ul \\nPrimary: bs-0292R, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \\nTMB staining; Read the data in MicroplateReader by 450nm. \\n
Lane 1: Human Serum lysates; Lane 2: Mouse Serum lysates; Lane 3: Rat Serum lysates; Lane 4: Goat Serum lysates; Lane 5: Cow Serum lysates probed with BSA Polyclonal Antibody, Unconjugated (bs-0292R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0292R

Applications: WB, ELISA, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Cow

Conjugate: Unconjugated

Catalog #1: bs-0292R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Cow

Conjugate: Cy3

Catalog #1: bs-0292R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Cow

Conjugate: FITC

Catalog #1: bs-0292R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Goat, Cow

Conjugate: Cy7

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CD79A\/IGBP-1 Polyclonal Antibody, Unconjugated (bs-0298R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-IGBP1 Polyclonal Antibody, Unconjugated (bs-0298R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with IGBP1 Polyclonal Antibody(bs-0298R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-0298R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0298R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bs-0298R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bs-0298R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bs-0298R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bs-0299R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0299R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-0299R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-0299R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-0299R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-0313R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0315R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0315R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bs-0315R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bs-0315R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Cy5

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ILK-1 Polyclonal Antibody (bs-0317R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 human colon carcinoma lysates probed with Anti ILK-1 Polyclonal Antibody, Unconjugated (bs-0317R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 50kD. Observed band size:50kD.\\n
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-ILK-1 Polyclonal Antibody (bs-0317R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-ILK-1 Polyclonal Antibody (bs-0317R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n

Catalog #1: bs-0317R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0315R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: FITC

Catalog #1: bs-0317R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-0317R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-0317R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-0317R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-0318R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0318R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-0318R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0318R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0318R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-0319r

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bs-0319R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Cy7

Catalog #1: bs-0319R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Cy3

Catalog #1: bs-0319R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: Cy5

Catalog #1: bs-0319R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus

Conjugate: FITC

Mouse thymus lysate probed with C5a anaphylatoxin Polyclonal Antibody, Unconjugated (bs-0324R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse thymus lysates probed with C5a anaphylatoxin Polyclonal Antibody, Unconjugated (bs-0324R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates probed with C5a Polyclonal Antibody, Unconjugated (bs-0324R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0324r

Applications: WB, ELISA, IHC-P, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0324r-cy3-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-0324r-cy5-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-0324r-fitc-100ul

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC