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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-0467R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-0467R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat liver lysates L2 mouse intestine lysates probed with Anti Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 25kD. Observed band size:25kD.
Mouse heart lysates probed with Rabbit Anti-Adiponectin Polyclonal Antibody, Unconjugated (bs-0471R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0471R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0474R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0474R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0474R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Formalin-fixed and paraffin embedded rat lung labeled with Anti- VSIG4 Polyclonal Antibody, Unconjugated (bs-0479R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Mouse lung and placenta lysates probed with VSIG4 Polyclonal Antibody, unconjugated (bs-0479R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-0479R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin-embedded human kidney tissue labeled with Rabbit Anti- IFN-gamma \\nPolyclonal Antibody, Unconjugated (bs-0481R) at 1:200 followed by conjugation to the secondary \\nantibody and DAB staining
HL-60 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Molt-4 lysates probed with IFN gamma Polyclonal Antibody, Unconjugated (bs-0481R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0481R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0481R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0481R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0483R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: HRP

Catalog #1: bs-0483R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-0483R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bs-0484R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-0484R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0484R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-0485R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti EphA2\/Eph receptor A2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat brain labeled with Anti-EphA2\/Eph receptor A2 Polyclonal Antibody, Unconjugated(bs-0485R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-EphA2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:100 followed by conjugation to the secondary antibody and DAB staining\\n
NIH\/3T3 cell lysates probed with EphA2 Polyclonal Antibody, Unconjugated (bs-0485R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0485R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0485R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse heart lysates probed with Anti CXorf36 Polyclonal Antibody, Unconjugated (bs-0488R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 20\/48kD. Observed band size:20kD.

Catalog #1: bs-0488R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0488R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0488R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0489R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Brdu Polyclonal Antibody (bs-0489R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. Hela(top) and MCF-7(bottom) cells were labeled with BrdU (10\u03bcM) and stained with Rabbit Anti-BrdU Polyclonal Antibody (bs-0489R) at 1:100 dilution. 2nd antibody without primary antibody was used as control included. Fluorescent signals were detected with primary antibody dilution in both Hela and MCF-7.

Catalog #1: bs-0489R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Species independent

Conjugate: Unconjugated

Catalog #1: bs-0489R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Species independent

Conjugate: Biotin

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-PPP4C Polyclonal Antibody, Unconjugated (bs-0490R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-PPP4C Polyclonal Antibody, Unconjugated (bs-0490R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse kidney lysates probed with PPP4C Polyclonal Antibody, Unconjugated (bs-0490R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PPP4C Polyclonal Antibody(bs-0490R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0490R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0490R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0490R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0492R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-RGS2 Polyclonal Antibody, Unconjugated (bs-0492R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-RGS2 Polyclonal Antibody, Unconjugated (bs-0492R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-0492R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0492R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-0494R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Xenopus laevis

Conjugate: Unconjugated

Catalog #1: bs-0493R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0494R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Xenopus laevis

Conjugate: Biotin

Catalog #1: bs-0494R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Xenopus laevis

Conjugate: HRP

Catalog #1: bs-0495R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-0495R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-0495R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-0496R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human breast carcinoma labeled with Anti-Dnmt3a Polyclonal Antibody, Unconjugated (bs-0497R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Dnmt3a Polyclonal Antibody, Unconjugated (bs-0497R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Image provided by One World Lab validation program. SH-SY5Y cells probed with Dnmt3a Polyclonal Antibody (bs-0497R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
U-87MG cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Dnmt3a Antibody(bs-R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0497R

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0497R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0497R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0500R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0500R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0500R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Formalin-fixed and paraffin embedded: human lung carcinoma labeled with Anti-JNK1\/3 Polyclonal Antibody (bs-0501R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-JNK1 + 3 Polyclonal Antibody at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with \\tJNK1 + 3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1+3 Polyclonal Antibody, Unconjugated (bs-0501R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0501R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0501R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0501R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP