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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2\/MAPKK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Images provided by the Independent Validation Program, badge number 029748. Lane one: HeLa cell lysates. Lane 2: c6\/36 Mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-MEK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250. Followed by Goat Anti-Rabbit antibody conjugated to Alexa fluor 488 at 1:500 dilution.

Catalog #1: bs-0223R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0223R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0223R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0224R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0224R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-0224R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-0225M

Applications: WB, ELISA, IHC-P

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0225M-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Others

Conjugate: Biotin

Catalog #1: bs-0225M-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Others

Conjugate: HRP

Catalog #1: bs-0226R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0226R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-0226R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-FGF1 Polyclonal Antibody, Unconjugated (bs-0229R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FGF1 Polyclonal Antibody(bs-0229R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0229R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0229R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0229R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded: rat colitis tissue labeled with Anti-PDGFRA Polyclonal Antibody, Unconjugated (bs-0231R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
SH-Sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDGFRA Antibody (bs-0231R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0231R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0231R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-0231R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-0232R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bs-0232R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded Human brain glioma labeled with Anti-PDGFRB Polyclonal Antibody, Unconjugated (bs-0232R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
DU145 lysates probed with PDGF Receptor beta Polyclonal Antibody, Unconjugated (bs-0232R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U251 lysates probed with PDGF Receptor beta Polyclonal Antibody, Unconjugated (bs-0232R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
NIH\/3T3 lysates probed with PDGF Receptor beta Polyclonal Antibody, Unconjugated (bs-0232R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0232R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-0235R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0235R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0235R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Mouse brain lysates probed with ChRM2 Polyclonal Antibody, Unconjugated (bs-0236R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Heart tissue lysates probed with ChRM2 Polyclonal Antibody, Unconjugated (bs-0236R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ChRM2) Polyclonal Antibody, Unconjugated (bs-0236R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0236R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0236R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0236R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C
L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha\/Beta\/Gamma\/Delta\/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 31kD. Observed band size: 31kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0237r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0242R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0242R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0242R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-0243R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Plant

Conjugate: Unconjugated

Catalog #1: bs-0243R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: Biotin

Catalog #1: bs-0243R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Plant

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-HSP70\/HSPA1A Polyclonal Antibody, Unconjugated (bs-0244R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with HSP70 1A\/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HSP70 Antibody(bs-0244R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Testis lysates; Lane 2: Mouse Epididymis lysates; Lane 3: Mouse Seminal vesicle lysates; Lane 4: Mouse Liver lysates; Lane 5: Mouse NIH\/3T3 cell lysates; Lane 6: Rat Testis lysates; Lane 7: Rat Spleen lysates; Lane 8: Human Hela cell lysates; Lane 9: Human Jurkat cell lysates; Lane 10: Human HepG2 cell lysates; Lane 11: Human A549 cell lysates; Lane 12: Human MCF-7 cell lysates probed with HSP70 1A\/1B Polyclonal Antibody, Unconjugated (bs-0244R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0244R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Unconjugated

Catalog #1: bs-0244R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: HRP

Catalog #1: bs-0244R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Pig, Chicken

Conjugate: Biotin

Catalog #1: bs-0245R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Mouse adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat adrenal gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRF Polyclonal Antibody, Unconjugated (bs-0246R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0246R

Applications: WB

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0247R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0247R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining
Mouse testis lysates probed with Rabbit Anti-EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.
Lane 1: Mouse Testis lysates; Lane 2: Rat Testis lysates; Lane 3: Human HUVEC cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Human A431 cell lysates; Lane 6: Human U-2OS cell lysates; Lane 7: Human MDA-MB-231 cell lysates; Lane 8: Human K562 cell lysates probed with EphB2 R Polyclonal Antibody, Unconjugated (bs-0247R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0247R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human esophageal carcinoma labeled with Rabbit Anti-PPAR delta Polyclonal Antibody, Unconjugated (bs-0250R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Testis tissue lysates probed with PPAR delta + beta Polyclonal Antibody, Unconjugated (bs-0250R) at 1:200 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0250R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-0250R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-0250R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-0251R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-0251R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-0251R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP