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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-9224R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9226R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-9226R-Biotin

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-9226R-HRP

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-9231R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9231R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9231R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9233R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9233R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9233R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9237R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9237R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9237R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9238R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9238R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9238R

Applications: ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9240R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9239R

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9240R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9240R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9241R

Applications: IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9241R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9241R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9243R-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-9243R-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-9243R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9244R-Biotin

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: Biotin

Catalog #1: bs-9244R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Mouse

Conjugate: Unconjugated

Catalog #1: bs-9244R-HRP

Applications: WB, IHC-P

Reactivity: Mouse

Conjugate: HRP

Catalog #1: bs-9248R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Lane 1: Mouse Liver lysates probed with RNF113A Polyclonal Antibody, Unconjugated (bs-9248R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (bs-9248R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (bs-9248R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (RNF113A) Polyclonal Antibody, Unconjugated (bs-9248R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-9248R

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9248R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9249R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9249R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-RNF123 Polyclonal Antibody, Unconjugated (bs-9249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Anti-RNF123 Polyclonal Antibody, Unconjugated (bs-9249R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse brain probed with Anti RNF123 Polyclonal Antibody, Unconjugated (bs-2530R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with RNF123 Polyclonal Antibody(bs-9249R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).

Catalog #1: bs-9249R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9250R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9250R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9250r-100ul

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9252R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9252R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9252R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9254R-Biotin

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-9253R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RNF138 Polyclonal Antibody, Unconjugated (bs-9254R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-9254R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-9254R-HRP

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-9257R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-9257R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Lovo cell lysates probed with RNF150 Polyclonal Antibody, Unconjugated (bs-9257R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Heart tissue lysates; Lane 2: Human Jurkat cell lysates probed with RNF150 Polyclonal Antibody, Unconjugated (bs-9257R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9257R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated