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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-10529R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Catalog #1: bs-10529R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-1052R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1054R

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1053R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Rat, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti Neurocan Polyclonal Antibody, Unconjugated (bs-1055R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Positive sample: Paraformaldehyde-fixed, paraffin embedded (rat brain, left); Negative sample: Paraformaldehyde-fixed, paraffin embedded (mouse liver, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Neurocan Polyclonal Antibody, Unconjugated (bs-1055R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining. \r\n
Positive sample\uff1aParaformaldehyde-fixed, paraffin embedded (mouse brain, left); Negative sample\uff1aParaformaldehyde-fixed, paraffin embedded (mouse liver, right); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Neurocan) Polyclonal Antibody, Unconjugated (bs-1055R) at 1:200 overnight at 4\u00b0C, followed by secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Neurocan) Polyclonal Antibody, Unconjugated (bs-1055R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-1055R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1055R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1055R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti 5-HTR2A\/HTR2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 human colon carcinoma lysates probed (bs-1056R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 52kDa.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 5-HT2A Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary for 20 minutes and DAB staining.
Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates probed with 5-HTR2 Polyclonal Antibody, Unconjugated (bs-1056R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1056r

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1056r-hrp-100ul

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1057R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat pancreas labeled with Rabbit Anti Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (bs-1057R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody, Unconjugated (bs-1057R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody (bs-1057R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-Integrin Alpha 3 + Beta 1 Polyclonal Antibody (bs-1057R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-1057R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1057R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1058R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and rat brain tissue labeled with Anti-ADRA2 Polyclonal Antibody, Unconjugated (bs-1062R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates, L2 rat brain lysates probed (bs-1062R) Anti-ADRA2 Polyclonal, Unconjugated at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 50kD.

Catalog #1: bs-1062R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-1062R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-1062R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti KLF4 Polyclonal Antibody, Unconjugated (bs-1064R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat kidney lysates L2 human colon carcinoma lysates probed with Anti KLF4 Polyclonal Antibody, Unconjugated (bs-1064R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 56kD. Observed band size: 56kD.

Catalog #1: bs-1064R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1064R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1064R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10656R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Mouse Thymus lysates probed with PI3 Kinase p110 delta Polyclonal Antibody, Unconjugated (bs-10656R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human HT-1080 cell lysates; Lane 2: Human Jurkat cell lysates probed with PI3 Kinase p110 delta Polyclonal Antibody, Unconjugated (bs-10656R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-10656R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-10656R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Formalin-fixed and paraffin embedded mouse embryo labeled with Rabbit Anti NGX6\/C9orf127 Polyclonal Antibody, Unconjugated (bs-1065R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 mouse liver lysates L2 mouse brain lysates probed with Anti NGX6\/C9orf127 Polyclonal Antibody, Unconjugated (bs-1065R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 52kD. Observed band size:52kD,42kD,25kD\\n

Catalog #1: bs-1065R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1065R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1065R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-1066R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1066R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1066R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1069R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1069R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-1069R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Formalin-fixed and paraffin embedded rat brain labeled with Anti-NPY1R Polyclonal Antibody, Unconjugated (bs-1070R) at 1:400 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded rat heart labeled with Anti-NPY1R Polyclonal Antibody, Unconjugated(bs-1070R) 1:400, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37\u00b0C.
Lane 1: Mouse NIH\/3T3 tissue lysates; Lane 2: Rat Cerebrum tissue lysates; Lane 3: Rat Kidney tissue lysates; Lane 4: Human 293T cell lysates probed with NPY1R Polyclonal Antibody, Unconjugated (bs-1070R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1070R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1071R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1071R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1071R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-10723R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-10723R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-10723R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: Biotin

Formalin-fixed and paraffin embedded rat small intestine labeled with Anti-Egr1 Polyclonal Antibody, Unconjugated (bs-1076R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse Lung lysates probed with Egr1 Polyclonal Antibody, Unconjugated (bs-1076R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.01%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Egr1 Polyclonal Antibody(bs-1076R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Egr1) polyclonal Antibody, Unconjugated (bs-1076R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bs-1076R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1076R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1076R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Lane 1: mouse embryo lysates Lane 2: human colon carcinoma lysates probed with Anti OCT2 Polyclonal Antibody, Unconjugated (bs-1077R) at 1:200 in 4C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37C. Predicted band 51kD. Observed band size: 51kD
Lane 1: A549 cell lysates probed with Rabbit Anti-OCT2 Polyclonal Antibody, Unconjugated (bs-1077R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
HeLa lysates probed with Rabbit Anti-OCT2 Polyclonal Antibody, Unconjugated (bs-1077R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.
Lane 1: Mouse Lymph node tissue lysates; Lane 2: Rat Lymph node tissue lysates probed with OCT2 Polyclonal Antibody, Unconjugated (bs-1077R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-1077R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1077R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1077R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1080R

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-1078R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-1080R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP