Free Shipping On Orders Over $1,000!

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Primary Antibodies

Collection Menu
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP)\r\nLysate: 1: HeLa, 2: 293, 3: A20\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 30 s\r\nPredicted MW: 39 kDa\r\nObserved MW: 39 kDa
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60554R
Tissue: mouse spleen\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:100\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60554R
Lane 1: Human Hela cell Lysates; Lane 2: Human 293T cell Lysates. Probed with Aurora B monoclonal Antibody, unconjugated (bsm-60554R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60554R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP)\r\nLysate: HeLa\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 18 kDa
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD59) Monoclonal Antibody, Unconjugated (bsm-60603R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD59) Monoclonal Antibody, Unconjugated (bsm-60603R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD59) Monoclonal Antibody, Unconjugated (bsm-60603R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bsm-60603R

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at\r\nroom temperature\r\nSecondary ab: Goat Anti-Rabbit IgG H&L\r\n(HRP)\r\nLysate: Jurkat\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 20 kDa\r\nObserved MW: 18-28 kDa
Tissue: Human tonsil\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60390R
Tissue: Human colon\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nSecondary ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-60390R
Lane 1: Human Jurkat cell Lysates; Lane 2: Human MOLT4 cell Lysates. Probed with CD3G monoclonal Antibody, unconjugated (bsm-60390R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-60390R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Tissue:Rat stomach\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60279R
Tissue:Rat liver\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60279R
Tissue:mouse stomach\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60279R
Tissue:Human breast cancer\r\nSection type: Formalin fixed & Paraffin -embedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000\r\nPrimary ab incubation condition: 1 hour at room temperature\r\nCounter stain: Hematoxylin\r\nComment: Color brown is the positive signal for bsm-60279R

Catalog #1: bsm-60279R

Applications: IHC-P

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-60078M

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:5000\r\nPrimary ab incubation condition: room temperature 2h\r\nSecondary ab: Goat Anti-Rabbit IgG H&L (HRP)\r\nLysate: 1: HeLa, 2: Panc-1, 3: PC-12, 4: NIH\/3T3\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 10 s\r\nPredicted MW: 45 kDa\r\nObserved MW: 45 kDa\r\n
Cell line: NIH-3T3\r\nFixative: 100% Ice-cold methanol\r\nPermeabilization: 0.1% TritonX-100\r\nPrimary ab dilution: 1:50\r\nPrimary incubation condition: 4\u00b0C overnight\r\nSecondary ab: Goat Anti-Rabbit IgG\r\nNuclear counter stain: DAPI (Blue)\r\nComment: Color green is the positive signal for bsm-60441R

Catalog #1: bsm-60441R

Applications: WB, IHC-P, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:2000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: (-) HeLa, (+) HeLa+Trichostatin A (2\u03bcM, 4hr)\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa\r\n
Blocking buffer: 5% NFDM\/TBST\r\nPrimary ab dilution: 1:1000\r\nPrimary ab incubation condition: 2 hours at room temperature\r\nSecondary ab: Goat Anti-Mouse IgG H&L (HRP)\r\nLysate: N2a, Mouse kidney, Rice\r\nProtein loading quantity: 20 \u03bcg\r\nExposure time: 60 s\r\nPredicted MW: 14 kDa\r\nObserved MW: 14 kDa\r\n

Catalog #1: bsm-60061M

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-60608R

Applications: IHC-P

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-30092M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30092M-A594

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-30092M-A555

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-30092M-A647

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-30092M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30092M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30092M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30092M-A488

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-30092M-A350

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-30092M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30092M-A750

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-30094M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-30092M-A680

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-30092M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30094M-A594

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-30092M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30094M-A647

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-30094M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30094M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30094M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30094M-A488

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-30094M-A555

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-30094M-A350

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-30094M-A680

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-30094M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30094M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30094M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30095M-A350

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-30095M-A594

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-30095M-A647

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-30095M-A488

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-30095M-A555

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-30095M-Cy3

Applications: FCM

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-30095M-Cy5.5

Applications: FCM

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-30095M-Cy5

Applications: FCM

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-30095M-FITC

Applications: FCM

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-30095M-PE

Applications: FCM

Reactivity: Human

Conjugate: PE

Catalog #1: bsm-30094M-A750

Applications: FCM

Reactivity: Human

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-30095M-Cy7

Applications: FCM

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-30095M

Applications: FCM

Reactivity: Human

Conjugate: Unconjugated