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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Formalin-fixed and paraffin embedded rat uterine myoma labeled with Rabbit Anti-ER-beta Polyclonal Antibody (bs-0166R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat uterine myoma labeled with Rabbit Anti-ER-beta Polyclonal Antibody (bs-0166R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Raw246.7 Cell lysates probed with Neurokinin B receptor Polyclonal Antibody, unconjugated (bs-0166R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
RSC96 cells probed with Neurokinin B receptor Antibody, unconjugated (bs-0166R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0166R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse embryo labeled with Anti-GFRA4 Polyclonal Antibody, Unconjugated (bs-0168R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human thyroid labeled with Anti-GFRA4 Polyclonal Antibody, Unconjugated(bs-0168R) 1:200, overnight at 4\u00b0C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37\u00b0C.

Catalog #1: bs-0168R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-VEGFR1\/FLT1 Polyclonal Antibody, Unconjugated (bs-0170R) at 1:400 followed by conjugation to the secondary antibody and DAB staining
Image kindly submitted by Victor Blanco as part of the Free Sample Program. Frozen mouse brain probed with bs-0170R at 1:100, overnight at 4C.
Mouse spleen cells probed with VEGFR1 Polyclonal Antibody, unconjugated (bs-0170R) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

Catalog #1: bs-0170R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat skeletal muscle labeled with Anti-IRS2 Polyclonal Antibody (bs-0173R), Unconjugated at 1:200followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Rabbit Anti IRS-2 Polyclonal Antibody, Unconjugated (bs-0173R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-0173R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-ER-alpha\/beta Polyclonal Antibody, Unconjugated (bs-0174R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain, L2 rat colon lysates probed (bs-0174R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 58kDa.
Tissue\/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (Estrogen Receptor alpha + beta ) Polyclonal Antibody, Unconjugated (bs-0174R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Estrogen Receptor alpha + beta Polyclonal Antibody, Unconjugated (bs-0174R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0174R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human nasopharyngeal carcinoma labeled with Anti-Trk-B Polyclonal Antibody (bs-0175R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
L1 rat brain lysates L2 rat kidney lysates probed with Anti Trk B\/NTRK2 Polyclonal Antibody, Unconjugated (bs-0175R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 90kD. Observed band size:90kD.\\n
RSC96 cells probed with TrkB Polyclonal Antibody, Unconjugated (bs-0175R) at [CNC.] for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human U-87MG cell lysates probed with TrkB Polyclonal Antibody, Unconjugated (bs-0175R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0175R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat sciatic nerve labeled with Anti-AGER\/RAGE Polyclonal Antibody, Unconjugated (bs-0177R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
NIH\/3T3 cells probed with Rabbit Anti-RAGE Polyclonal Antibody (bs-0177R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.\\n
Raji Cells lysates probed with RAGE Polyclonal Antibody, unconjugated (bs-0177R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with RAGE Polyclonal Antibody(bs-0177R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0177R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PSD-95 Polyclonal Antibody (bs-0179R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse Cerebellum tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat Cerebellum tissue lysates; Lane 5: Human U87MG cell lysates; Lane 6: Human SH-SY5Y cell lysates probed with PSD95 Polyclonal Antibody, Unconjugated (bs-0179R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0179R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti DDC\/DOPA Decarboxylase Polyclonal Antibody, Unconjugated (bs-0180R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Mouse adrenal gland tissue lysates; Lane 3: Rat Cerebrum tissue lysates; Lane 4: Rat adrenal gland tissue lysates ; Lane 5: Human SH-SY5Y cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human Jurkat cell lysates; Lane 8: Human A549 cell lysates probed with DOPA Decarboxylase Polyclonal Antibody,Unconjugated (bs-0180R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0180R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-Nociceptin receptor Polyclonal Antibody (bs-0181R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-Nociceptin receptor Polyclonal Antibody (bs-0181R) at 1:200 followed by conjugation to the secondary antibody and DAPI staining.
Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-Nociceptin receptor Polyclonal Antibody (bs-0181R) at 1:200 followed by conjugation to the secondary antibody.
L1 mouse brain, L2 mouse kidney lysates probed (bs-0181R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 40kDa.

Catalog #1: bs-0181R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human endometrium tissue labeled with Anti-PTP-1B Polyclonal Antibody (bs-0182R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 cell lysates probed with Rabbit Anti-PTP1B Polyclonal Antibody, Unconjugated (bs-0182R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-PTP1B Polyclonal Antibody (bs-0182R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-PTP1B Polyclonal Antibody (bs-0182R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Catalog #1: bs-0182R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat transplant lymphoma labeled with Anti-PDGF-B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysates probed with Rabbit Anti-PDGF-B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Lane 1: Mouse Kidney lysates; Lane 2: Mouse Cerebrum lysates; Lane 3: Rat Cerebrum lysates; Lane 4: Mouse Lung lysates; Lane 5: Rat Lung lysates; Lane 6: Human HUVEC cell lysates; Lane 7: Human A431 cell lysates; Lane 8: Human MCF-7 cell lysates; Lane 9: Human U-2OS cell lysates probed with PDGF B Polyclonal Antibody, Unconjugated (bs-0185R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0185R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung inflammation labeled with Rabbit Anti IRS-3 Polyclonal Antibody, Unconjugated (bs-0186R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Rat adipose lysates probed with IRS-3 Polyclonal Antibody, Unconjugated (bs-0186R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0186R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-0187R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat kidney tissue labeled with Anti-ET-1 Polyclonal Antibody (bs-0188R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat lung labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat stomach labeled with Anti- Preproendothelin 1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat heart muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Big endothelin-1 Polyclonal Antibody, Unconjugated (bs-0188R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0188r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Rat cerebrum lysates probed with PACAP-27\/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat cerebrum lysates probed with PACAP-27\/38 Polyclonal Antibody, Unconjugated (bs-0190R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0190R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti Trk A\/B\/C Polyclonal Antibody, Unconjugated (bs-0192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Trk A\/B\/C Polyclonal Antibody, Unconjugated (bs-0192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human K562 cell lysates probed withTyrosine Kinase Receptor Polyclonal Antibody, Unconjugated (bs-0192R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Mouse Large intestine lysates; Lane 4: Mouse Uterus lysates probed with Tyrosine Kinase Receptor Polyclonal Antibody, Unconjugated (bs-0192R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0192R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti CYP5A1\/Thromboxane synthase Polyclonal Antibody, Unconjugated (bs-4019R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti HSP60 Polyclonal Antibody, Unconjugated (bs-0191R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
MCF-7 Lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Mouse liver lysates probed with Rabbit Anti-HSP60 Polyclonal Antibody (bs-0191R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP ) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0191R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human endometrium Anti-TrK-A\/NTRK1 Polyclonal Antibody (bs-0193R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TrkA Polyclonal Antibody, Unconjugated (bs-0193R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse Brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TrkA) Polyclonal Antibody, Unconjugated (bs-0193R) at 1:400 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-Cy3) for 90 minutes, and DAPI for nuclei staining.
U251 lysates probed with TrkA Polyclonal Antibody, Unconjugated (bs-0193R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0193R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

L1 human colon carcinoma lysates, L2 mouse liver lysates probed with Anti GRP94\/HSP GP96 Polyclonal Antibody, Unconjugated (bs-0194R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 86kD
Mouse placenta lysates probed with Rabbit Anti-GRP94 Polyclonal Antibody, Unconjugated (bs-0194R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at RT
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GRP94 Polyclonal Antibody(bs-0194R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GRP94 Polyclonal Antibody(bs-0194R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0194R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human colon tubular adenocarcinoma labeled with Anti-PDGF-A Polyclonal Antibody (bs-0196R), Unconjugated at 1:300, followed by conjugation to the secondary antibody and DAB staining
Mouse lung lysate probed with Anti-PDGF-A Polyclonal Antibody (bs-0196R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.
Mouse placenta lysate probed with Anti-PDGF-A Polyclonal Antibody (bs-0196R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.

Catalog #1: bs-0196R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti VIP receptor 2\/VPAC2 Polyclonal Antibody, Unconjugated (bs-0197R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A431 lysates probed with VPAC2R Polyclonal Antibody, Unconjugated (bs-0197R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0197R

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt

Reactivity: Human, Rat

Conjugate: Unconjugated

Mouse brain lysates and Human A549 cells probed with PACAP receptor 1 Polyclonal Antibody, unconjugated (bs-0198R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with PACAP receptor Polyclonal Antibody, Unconjugated (bs-0198R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0198R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-PACAP-38 Polyclonal Antibody (bs-0200R) , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Rabbit Anti-PACAP-38 Polyclonal Antibody (bs-0200R) , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Rabbit Anti-PACAP-38 Polyclonal Antibody (bs-0200R) , Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human kidney labeled with Anti-PACAP-38 Polyclonal Antibody, Unconjugated (bs-0200R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Catalog #1: bs-0200R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-GDNFRA Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GDNFRA) Polyclonal Antibody, Unconjugated (bs-0201R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0201R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ET-1 Polyclonal Antibody, Unconjugated (bs-0202R) at 1:200 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Testis tissue lysates; Lane 2: Rat Testis tissue lysates probed with Big endothelin-1 Polyclonal Antibody, Unconjugated (bs-0202R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0202r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0203R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GHRH Polyclonal Antibody (bs-0205R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse brain labeled with Anti-GHRH Polyclonal Antibody, Unconjugated (bs-0205R) at 1:300 followed by conjugation to the secondary antibody (bs-0295G-HRP) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (GHRH) Polyclonal Antibody, Unconjugated (bs-0205R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0205R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung tissue labeled with Rabbit Anti-TSHR Polyclonal Antibody (bs-0206R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Mouse thymocytes probed with Rabbit Anti-TSHR Polyclonal Antibody, Unconjugated (bs-0206R) (green) at 1:100 for 40 minutes followed by a FITC conjugated secondary compared to control cells (blue).
Lane 1: Human Jurkat cell lysates; Lane 2: Human TT cell lysates probed with TSHR (CT) Polyclonal Antibody, Unconjugated (bs-0206R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0206r

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human stomach tissue labeled with Anti-GLP-2 Polyclonal Antibody, Unconjugated (bs-0208R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed U2OS cells; Incubated by 0.3% Triton-100 for 20 min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GLP-2 Polyclonal Antibody, Unconjugated (bs-0208R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody (bs-0295G-FITC) for 90 minutes and DAPI staining.

Catalog #1: bs-0208R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Tubulin-beta Polyclonal Antibody, Unconjugated (bs-0210R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
RSC96 cells probed with Tubulin-beta Polyclonal Antibody, Unconjugated (bs-0210R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Lane 1: Mouse Cerebellum Lysate;Lane 2: Rat Cerebellum Lysate;Lane 3: Mouse Cerebrum Lysate;Lane 4: Rat Cerebrum Lysate;Lane 5:Mouse Ovary Lysate;Lane 6:Mouse Heart Lysate;Lane 7: Rat Heart Lysate probed with beta Tubulin Polyclonal Antibody, Unconjugated (bs-0210R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with beta Tubulin Polyclonal Antibody(bs-0210R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0210R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Pig

Conjugate: Unconjugated

Catalog #1: bs-0211R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0214R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-Fas Polyclonal Antibody, Unconjugated (bs-0215R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Thymus tissue lysates; Lane 2: Rat Thymus tissue lysates; Lane 3: Human HepG2 cell Lysates; Lane 4: Human Raji cell Lysates; Lane 5: Human HT1080 cell Lysates; Lane 6: Human A431 cell Lysates probed with CD95\/FAS Polyclonal Antibody, Unconjugated (bs-0215R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.\r\n

Catalog #1: bs-0215R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-FasL Polyclonal Antibody, Unconjugated (bs-0216R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Jurkat lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Bone lysates probed with Fas Ligand Polyclonal Antibody, Unconjugated (bs-0216R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0216R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0221R

Applications: WB

Reactivity: Human, Virus, Others

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti MEK2\/MAPKK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Images provided by the Independent Validation Program, badge number 029748. Lane one: HeLa cell lysates. Lane 2: c6\/36 Mosquito cell extract (non-reactive\\nspecies) probed with Rabbit Anti-MEK2 Polyclonal Antibody, Unconjugated (bs-0223R) at 1:100 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:20000 for 60 min at 26˚C.
HT-55 cells were stained with bs-0223R Rabbit Anti-MEK2 Polyclonal Antibody at 1:250. Followed by Goat Anti-Rabbit antibody conjugated to Alexa fluor 488 at 1:500 dilution.

Catalog #1: bs-0223R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0224R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-0225M

Applications: WB, ELISA, IHC-P

Reactivity: Others

Conjugate: Unconjugated

Catalog #1: bs-0226R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse kidney labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FGF1\/AFGF Polyclonal Antibody, Unconjugated (bs-0229R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with Rabbit Anti-FGF1 Polyclonal Antibody, Unconjugated (bs-0229R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
A431 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FGF1 Polyclonal Antibody(bs-0229R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0229R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat colitis tissue labeled with Anti-PDGFRA Polyclonal Antibody, Unconjugated (bs-0231R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
SH-Sy5y (Positive) and A431 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDGFRA Antibody (bs-0231R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0231R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded Human brain glioma labeled with Anti-PDGFRB Polyclonal Antibody, Unconjugated (bs-0232R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
DU145 lysates probed with PDGF Receptor beta Polyclonal Antibody, Unconjugated (bs-0232R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
U251 lysates probed with PDGF Receptor beta Polyclonal Antibody, Unconjugated (bs-0232R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
NIH\/3T3 lysates probed with PDGF Receptor beta Polyclonal Antibody, Unconjugated (bs-0232R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Catalog #1: bs-0232R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bs-0234R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: rat brain tissue labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat spinal cord labeled with Anti-PMP22 Polyclonal Antibody, Unconjugated (bs-0235R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n

Catalog #1: bs-0235R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Mouse brain lysates probed with ChRM2 Polyclonal Antibody, Unconjugated (bs-0236R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Mouse Spinal cord tissue lysates; Lane 2: Mouse Heart tissue lysates; Lane 3: Rat Spinal cord tissue lysates; Lane 4: Rat Heart tissue lysates probed with ChRM2 Polyclonal Antibody, Unconjugated (bs-0236R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ChRM2) Polyclonal Antibody, Unconjugated (bs-0236R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-0236R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37\u00b0C
L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha\/Beta\/Gamma\/Delta\/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 31kD. Observed band size: 31kD.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with 14-3-3 Alpha + Beta + Gamma + Delta + Epsilon Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0237r

Applications: WB, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-0242R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated