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Lane 1: A431; Lane 2: HepG2; Lane 3: Hela lysate probed with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HeLa cells with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
IF(ICC) staining with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MAP2K1 (7D8) Monoclonal Antibody (bsm-52311R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #: bsm-52311R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Catalog #: bs-23444R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: HRP

Catalog #: bs-23444R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-23444R-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-23444R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: Biotin

Catalog #: bs-23444R-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-23444R-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-23444R-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: FITC

Catalog #: bs-23444R-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-23444R-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: Cy7

Catalog #: bs-23444R-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: Cy5

Catalog #: bs-23444R-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: Cy3

Catalog #: bs-23444R-A680

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-23444R-A750

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-23444R-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit, Horse

Conjugate: Cy5.5

Catalog #: bs-8721R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #: bs-8721R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Biotin

Catalog #: bs-8721R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: HRP

Catalog #: bs-8721R-A350

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-8721R-A555

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-8721R-A488

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-8721R-A594

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-8721R-A647

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-8721R-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy3

Catalog #: bs-8721R-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy5.5

Catalog #: bs-8721R-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy5

Catalog #: bs-8721R-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: FITC

Catalog #: bs-8721R-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: Cy7

Catalog #: bs-8721R-A680

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-8747R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Biotin

Catalog #: bs-8721R-A750

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-8747R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: HRP

Catalog #: bs-8747R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Unconjugated

Catalog #: bs-8747R-A350

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-8747R-A488

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-8747R-A555

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-8747R-A594

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-8747R-A647

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-8747R-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy3

Catalog #: bs-8747R-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy5

Catalog #: bs-8747R-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy7

Catalog #: bs-8747R-Cy5.5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: Cy5.5

Catalog #: bs-8747R-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: FITC

Catalog #: bs-8747R-A680

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-8747R-A750

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Sheep, Rabbit, Horse

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-9439R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Biotin

Formalin-fixed and paraffin embedded human bladder carcinoma labeled with Rabbit Anti FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-9439P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FOXO1A Polyclonal Antibody(bs-9439R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Rat Lymph node lysates; Lane 2: Rat Bone lysates; Lane 3: Rat Liver lysates probed with FOXO1A Polyclonal Antibody, Unconjugated (bs-9439R) at 1:1000 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Catalog #: bs-9439R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Catalog #: bs-9439R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Chicken

Conjugate: HRP