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Catalog #: bs-1019R-A488

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 488

Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat ovary tissue labeled with Rabbit Anti-TLR2\/CD282 Polyclonal Antibody (bs-1019R), Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining
Human A549 cells probed with Rabbit Anti-TLR2 Polyclonal Antibody, Unconjugated (bs-1019R) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
Mouse spleen cells probed with \\tTLR2 Polyclonal Antibody, Unconjugated (bs-1019R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

Catalog #: bs-1019R

Applications: WB, FCM, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #: bs-1019R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #: bs-1019R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #: bs-1019R-A350

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-1019R-Cy3

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #: bs-1019R-A555

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-1019R-A594

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-1019R-A647

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-1019R-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5

Catalog #: bs-1019R-A750

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-1019R-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

Catalog #: bs-1019R-FITC

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #: bs-1019R-A680

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-1019R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: PE

Catalog #: bs-1782R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Bovine, Dog, Pig, Chicken

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4\u02daC. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37\u02daC. Predicted and observed band size: 17kDa,33kD
Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Catalog #: bs-0081R

Applications: WB, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: Unconjugated

Catalog #: bs-0081R-A350

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-0081R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: HRP

Catalog #: bs-0081R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: Biotin

Catalog #: bs-0081R-Cy7

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: Cy7

Catalog #: bs-0081R-A555

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-0081R-A594

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-0081R-A488

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-0081R-Cy5

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: Cy5

Catalog #: bs-0081R-A647

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-0081R-Cy3

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: Cy3

Catalog #: bs-0081R-A750

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-0081R-FITC

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: FITC

Catalog #: bs-0081R-A680

Applications: IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Goat

Conjugate: ALEXA FLUOR® 680

L1 rat liver lysates L2 rat kidney lysates probed with Rabbit Anti-ERK1 (Thr203\/Tyr205) + ERK2 (Thr183\/Tyr185) Polyclonal Antibody, Unconjugated (bs-1522R) at 1:200 overnight at 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37\u02daC. Predicted band 42kD. Observed band size: 42kD
Formalin-fixed and paraffin embedded mouse embryo tissue labeled with Rabbit Anti-ERK1 (Thr203\/Tyr205) + ERK2 (Thr183\/Tyr185) Polyclonal Antibody, Unconjugated (bs-1522R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Tissue\/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-ERK1 + 2 (Thr183\/Tyr185)) polyclonal Antibody, Unconjugated (bs-1522R) 1:100, 90 minutes at 37\u00b0C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue\/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (phospho-ERK1 + 2 (Thr183\/Tyr185)) Polyclonal Antibody, Unconjugated (bs-1522R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #: bs-1522R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Unconjugated

Catalog #: bs-1522R-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-1522R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Biotin

Catalog #: bs-1522R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: HRP

Catalog #: bs-1522R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-1522R-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-1522R-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-1522R-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-1522R-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy3

Catalog #: bs-1640R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-1522R-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: FITC

Catalog #: bs-1522R-A750

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-1522R-A680

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-1522R-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy7

L1 mouse brain lysates L2 human colon carcinoma lysates probed with Anti - phospho-JNK1\/2\/3 (Thr183+Tyr185) Polyclonal Antibody, Unconjugated (bs-1640R) at 1:200 in 4\u02daC. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37\u02daC. Predicted band 42kD. Observed band size: 42kD
Image kindly provided by Dr. Magdalena Krol. Control tumor cells, tumor cells grown in macrophage-conditioned medium, tumor cells sorted from co-culture with macrophages, and macrophages from monocultures and sorted from co-culture with tumor cells were analyzed. Total protein concentrations in lysates were determined using a Bio-Rad protein assay. Proteins (50 mg) were resolved using SDS-PAGE and transferred onto PVDF membranes. The membranes were then blocked with 5% non-fat dry milk in TBS buffer containing 0.5% Tween 20. The membranes were then incubated overnight with the primary Rabbit Anti-JNK1+2+3 (THR183+TYR185) at 1:100 dilution. Subsequently, the membranes were washed three times in TBS containing 0.5% Tween 20 and incubated for 1 h at room temperature with secondary antibodies conjugated with the appropriate infrared (IR) fluorophore IRDyeH 800 CW or IRDyeH 680 RD at a dilution of 1:5000.
Mouse splenocytes probed with Rabbit Anti-JNK1\/2\/3 (Thr183+Tyr185) Polyclonal Antibody, Unconjugated (bs-1640R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with JNK1+2+3 (T183+Y185) Polyclonal Antibody, Unconjugated (bs-1640R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #: bs-1640R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Unconjugated

Catalog #: bs-1640R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: HRP

Catalog #: bs-1640R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog

Conjugate: Biotin

Catalog #: bs-1522R-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat, Bovine

Conjugate: Cy5