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Primary Antibodies

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Catalog #1: bs-0468R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 555

Formalin-fixed: Endothelial cells labeled with Anti-CD31\/PECAM-1 Polyclonal Antibody, Unconjugated (bs-0468R) at 1:200, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC) at 1:200 for 40 minutes at 37\u00b0
Rat spleen cells probed with Rabbit Anti-CD31 Polyclonal Antibody (bs-0468R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CD31 Polyclonal Antibody, Unconjugated (bs-0468R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Recombinant human CD31 protein probed with CD31 Polyclonal Antibody, unconjugated (bs-0468R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.

Catalog #1: bs-0468R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0468R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0468R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0468R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: FITC

Catalog #1: bs-0468R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-0468R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0468R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: Cy3

Catalog #1: bs-0468R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: Cy5

Catalog #1: bs-0468R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: Cy7

Catalog #1: bs-0468R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: Biotin

Catalog #1: bs-0468R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: PE

Catalog #1: bs-0468R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-0468R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-0468R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit

Conjugate: HRP

Mouse splenocytes probed with Rabbit Anti-IGF1R Polyclonal Antibody (bs-0227R ) at 1:100 for 30 minutes followed by incubation with Anti-Rabbit IgG FITC conjugated secondary for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R Antibody(bs-0227R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
MCF7 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with IGF1R Polyclonal Antibody(bs-0227R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-0227R

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0227R-A594

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-0227R-A350

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0227R-A488

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0227R-A555

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-0227R-Cy7

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0227R-Cy5

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0227R-A647

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0227R-FITC

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0227R-Cy3

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0227R-PE

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: PE

Catalog #1: bs-0227R-A750

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-0227R-A680

Applications: FCM

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-0014R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F, IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rat Carotid artery labeled with Rabbit Anti-IGF-I Polyclonal Antibody (bs-0014R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-IGF-1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IGF 1 Polyclonal Antibody, Unconjugated (bs-0014R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-0014R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), IP

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-0014R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0014R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0014R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0014R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-0014R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-0014R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-0014R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy5

Catalog #1: bs-0014R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy3

Catalog #1: bs-0014R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: FITC

Catalog #1: bs-0014R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-0014R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Cy7

Catalog #1: bs-0014R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-4609R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 488

Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-CD105 Polyclonal Antibody (bs-4609R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain, L2 rat heart lysates probed (bs-4609R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 70kDa.
Image provided by One World Lab validation program. U138 cells probed with Rabbit Rabbit Anti-CD105 Polyclonal Antibody (bs-4609R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\\n
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-4609R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Unconjugated

Catalog #1: bs-4609R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: Biotin

Catalog #1: bs-4609R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Dog

Conjugate: HRP

Catalog #1: bs-4609R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-4609R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog

Conjugate: ALEXA FLUOR® 647