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Catalog #1: bs-3494R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: HRP

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Phospho-FRAP1\/mTOR(Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 followed by conjugation to the secondary antibody and DAB stainin
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with mTOR (Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Hela cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with mTOR (Ser2448) Polyclonal Antibody(bs-3494R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR (Ser2448) Polyclonal Antibody, Unconjugated (bs-3494R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-3494R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: Unconjugated

Catalog #1: bs-3494R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: Biotin

Catalog #1: bs-3494R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: Cy3

Catalog #1: bs-3494R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: Cy5

Catalog #1: bs-3494R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: FITC

Catalog #1: bs-3494R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: Cy7

Catalog #1: bs-0876R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: HRP

Catalog #1: bs-0876R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Biotin

Formalin-fixed and paraffin embedded human endometrium tissue labeled with Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse tumor with labeled Anti-phospho-AKT\/PKB(Ser473) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:600, followed by conjugation to the secondary antibody and DAB staining
Mouse liver lysates probed with Rabbit Anti-AKT(Ser473) Polyclonal Antibody, Unconjugated (bs-0876) at 1:300 overnight at 4˚C. Incubate with HRP conjugated Goat-Anti-Rabbit IgG (bs-0295G-HRP) at 1: 5000 for 90min at 37˚C.
U937 cell lysates probed with Anti-AKT1\/2\/3 (Ser472\/Ser473\/Ser474) Polyclonal Antibody, Unconjugated (bs-0876R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Catalog #1: bs-0876R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Unconjugated

Catalog #1: bs-0876R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy3

Catalog #1: bs-0876R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy5

Catalog #1: bs-0876R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: FITC

Catalog #1: bs-0876R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy7

Formalin-fixed and paraffin-embedded rat colon labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded rabbit meniscus tissue labeled with Anti-Collagen I Polyclonal Antibody, Unconjugated (bs-0578R) 1:300 followed by conjugation to the secondary PE antibody and DAB staining
Paraformaldehyde-fixed, paraffin-embedded Human Lung Cancer tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with Pro-Collagen 1 Polyclonal Antibody, Unconjugated (bs-0578R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bs-0578R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Fish

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
L1 rat brain lysates, L2 rat heart lysates probed with Anti-Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band and observed band size: 42kD

Catalog #1: bs-1365R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Fish

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded mouse melanoma tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated(bs-1992R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat testis tissue labeled with Anti-mTOR Polyclonal Antibody, Unconjugated (bs-1992R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE)and DAPI staining
Paraformaldehyde-fixed, paraffin embedded Mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with mTOR\/FRAP Polyclonal Antibody, Unconjugated (bs-1992R) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bs-1992R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Unconjugated

Catalog #1: bs-1992R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: HRP

Catalog #1: bs-1992R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Biotin

Catalog #1: bs-1992R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: FITC

Catalog #1: bs-1992R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Cy5

Catalog #1: bs-1992R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Cy3

Catalog #1: bs-1992R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Cy7

Catalog #1: bs-7777R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy3

Catalog #1: bs-7777R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Fish

Conjugate: Biotin

Catalog #1: bs-7777R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Fish

Conjugate: HRP

Catalog #1: bs-7777R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Unconjugated

Catalog #1: bs-7777R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy5

Catalog #1: bs-7777R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: FITC

Catalog #1: bs-7777R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy7

Zebrafish lysate probed with Ferritin (7A5) Monoclonal Antibody (bsm-52548R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Ferritin (7A5) Monoclonal Antibody (bsm-52548R) at 1:100 in MCF-7 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with Ferritin (7A5) Monoclonal Antibody (bsm-52548R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52548R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Fish, Zebrafish

Conjugate: Unconjugated

Lane 1: HepG2; Lane 2: PC12; Lane 3: NIH\/3T3 probed with MAP2K1\/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with MAP2K1\/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with MAP2K1\/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with MAP2K1\/2 (4H7) Monoclonal Antibody (bsm-52280R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52280R

Applications: WB, FCM, IHC-P, IF(IHC-P), IF(ICC)

Reactivity: Human, Mouse, Rat, Fish, Zebrafish

Conjugate: Unconjugated

Lane 1: 293; Lane 2: Jurkat; Lane 3: Mouse kidney; Lane 4: HepG2 lysate probed with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HepG2 cells with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:50 dilution followed by secondary antibody incubation (red), compared with an unlabeled control (cells without incubation with primary antibody; black).
Hybrid fish (crucian-carp) liver tissue probed with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with Prohibitin (1F10) Monoclonal Antibody (bsm-52282R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52282R

Applications: WB, FCM, IHC-P, IHC-F, IF(ICC)

Reactivity: Human, Mouse, Rat, Fish, Zebrafish

Conjugate: Unconjugated

Catalog #1: bs-3494R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: Cy5.5

Catalog #1: bs-0876R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Rabbit, Fish, Others

Conjugate: Cy5.5

Catalog #1: bs-1992R-Cy5.5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: Cy5.5

Catalog #1: bs-7777R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy5.5

Catalog #1: bs-12778R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Fish

Conjugate: Biotin

Catalog #1: bs-12778R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Fish

Conjugate: HRP

Catalog #1: bs-12778R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Fish

Conjugate: Unconjugated

Catalog #1: bs-12778R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy3

Catalog #1: bs-12778R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy5

Catalog #1: bs-12778R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy5.5

Catalog #1: bs-12778R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: Cy7

Catalog #1: bs-12778R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Fish

Conjugate: FITC

Catalog #1: bs-3494R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: PE

Catalog #1: bs-1992R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Bovine, Chicken, Fish, Others

Conjugate: PE

Catalog #1: bs-3494R-BF350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Fish

Conjugate: AbBy Fluor® 350