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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-2442R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-3280R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2442R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SP-D\/PSPD Polyclonal Antibody, Unconjugated(bs-1538R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded mouse lung tissue labeled Anti-SP-D Polyclonal Antibody, Unconjugated (bs-1583R) at 1:2000, followed by conjugation to the secondary antibody and DAB staining
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with SP-D\/PSPD Antibody(bs-1583R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1583R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Kidney lysates; Lane 3: Rat Thymus lysates; Lane 4: Rat Lymph node lysates; Lane 5: Rat Kidney lysates probed with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with VEGF-C Polyclonal Antibody(bs-1586R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-1586R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1586R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1586R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1586R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-1586R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-1586R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-1586R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-4754R-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with VWF Polyclonal Antibody (bs-4754R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with VWF Polyclonal Antibody (bs-4754R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

Catalog #1: bs-4754R

Applications: FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4754R-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-0586R

Applications: IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-4754R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-4754R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-4754R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-4754R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Formalin-fixed and paraffin embedded rat brain tissue labeled with Rabbit Anti-EpCAM\/CD326 Polyclonal Antibody (bs-0593R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit bladder tissue labeled with Rabbit Anti-EpCAM\/CD326 Polyclonal Antibody (bs-0593R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rabbit bladder tissue labeled with Rabbit Anti-EpCAM\/CD326 Polyclonal Antibody (bs-0593R), Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining.
L1 mouse brain, L2 mouse liver lysates probed (bs-0593R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Observed band size: 32kDa.

Catalog #1: bs-0593R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rabbit

Conjugate: Unconjugated

Mouse heart lysates probed with Nox1 Polyclonal Antibody, Unconjugated (bs-3682R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse testis lysates; Lane 2: Mouse uterus lysates; Lane 3: DU145 cell lysates probed with Nox1 Polyclonal Antibody, Unconjugated (bs-3682R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-3682R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Expression of cell surface marker CD34 and CD133 detected by flow cytometry
Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated(bs-2038R) at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3), and DAPI staining
Lane 1: human colon carcinoma Lane 2: rat kidney lysates probed with Anti CD34 Polyclonal Antibody, Unconjugated (bs-2038R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 39kD. Observed band size: 39kD.
25 ug total protein per lane of various lysates (see on figure) probed with CD34 polyclonal antibody, unconjugated (bs-2038R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bs-2038r

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-2038r-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-2038r-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-13004R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-13004R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-13004R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-13004R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-13004R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-13004R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

lane 1: Mouse brain; Lane 2: Mouse spleen lysates probed with Rabbit Anti-TLR7 Polyclonal Antibody (bs-6601R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C
Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti-TLR7 Polyclonal Antibody, Unconjugated (bs-6601R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) DAPI staining
Lane 1: Human THP-1(LPS) cell lysates; Lane 2: Human THP-1 cell lysates; Lane 3: Human Jurkat cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human 293T cell lysates probed with TLR7 Polyclonal Antibody, Unconjugated (bs-6601R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6601R

Applications: WB, ELISA

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-13004R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

MCF-7 cells probed with Rabbit Anti-Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody (bs-1310R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary Antibody.
Human U937 probed with \\tIntegrin Alpha V + Beta 3 Antibody, Unconjugated (bs-1310R ) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
HUVEC cell lysates probed with Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody, Unconjugated (bs-1310R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human Invasive urothelial carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Integrin Alpha V + Beta 3 (CD51+CD61) Polyclonal Antibody, Unconjugated (bs-1310R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Catalog #1: bs-1310r

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-1310R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-1310R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-1310R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-1310R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-1310R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-1310R-PE

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: PE

Formalin-fixed and paraffin embedded cervical cancer labeled with Anti-Integrin \u03b1V+\u03b25 Polyclonal Antibody, Unconjugated (bs-1356R) followed by conjugation to the secondary antibody and DAB staining
A549 cells probed with Rabbit Anti-Integrin Alpha V + Beta 5 Polyclonal Antibody (bs-1356R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated Secondary.\\n
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-Integrin Alpha V + Beta 5 Polyclonal Antibody, Unconjugated (bs-1356R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Integrin Alpha V + Beta 5 Polyclonal Antibody, Unconjugated (bs-1356R) at 1:400 overnight at 4\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Catalog #1: bs-1356R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-1310R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-1356R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-1356R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-1356R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-1356R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-1356R-PE

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-1356R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-1356R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP