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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bs-6259R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-6259R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-6259R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity:

Conjugate: HRP

Catalog #1: bs-6259R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity:

Conjugate: FITC

Catalog #1: bs-6266R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6266R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-6266R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-6266R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-6266R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-6266R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-6266R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

HepG2 cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: Siha cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: HepG2 cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: K562 cell lysates; Lane 2: Hela cell lysates probed with MCK10 (Tyr513) Polyclonal Antibody, Unconjugated (bs-6269R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6269R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6269R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-6269R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti FUT8 Polyclonal Antibody, Unconjugated (bs-6280R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-FUT8 Polyclonal Antibody, Unconjugated (bs-6280R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-6280R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-6280R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-6280R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-6280R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-6280R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-6280R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-6280R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat

Conjugate: FITC

Formalin-fixed and paraffin embedded human liver carcinoma labeled with Rabbit Anti-Hepsin Polyclonal Antibody, Unconjugated (bs-6281R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
HepG2 Cell lysates probed with Hepsin Polyclonal Antibody, unconjugated (bs-6281R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Catalog #1: bs-6281R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6281R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-6281R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-6281R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-6281R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-6281R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-6281R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-6286R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-6286R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-6286R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6286R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-6286R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-6286R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bs-6286R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-BRD8 Polyclonal Antibody, Unconjugated (bs-6290R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Hela cell lysates; Lane 2: Siha cell lysates; Lane 3: Molt-4 cell lysates probed with BRD8 Polyclonal Antibody, Unconjugated (bs-6290R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-6290R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-6294R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6293R

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6294R-Biotin

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: Biotin

Catalog #1: bs-6294R-HRP

Applications: ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bs-53060R

Applications: ELISA, ChIP-seq

Reactivity: Human, Mouse

Conjugate: Unconjugated

To determine the titer, an ELISA was performed using a serial dilution of the EHMT1 antibody (bs-53068R). The wells were coated with peptides. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,000.
Nuclear extracts of HeLa cells (40 \u03bcg) were analyzed by Western blot using the EHMT1 antibody (bs-53068R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment may represent a splicing variant.

Catalog #1: bs-53068R

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bs-6294R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bs-6294R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bs-53077R

Applications: ELISA

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-6294R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bs-53080R

Applications: WB, ELISA

Reactivity: Human

Conjugate: Unconjugated

Nuclear extracts (40 \u03bcg) from HeLa cells were analysed by Western blot using the Bioss antibody against MeCP2 (Cat. No. bs-53112R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
Whole cell extracts (40 \u03bcg) from HeLa cells transfected with MeCP2 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the Bioss antibody against MeCP2 (Cat. No. bs-53112R) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Bioss antibody directed against MeCP2 (Cat. No. bs-53112R) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.
ChIP assays were performed using human osteosarcoma (U2OS) cells, MeCP2 Polyclonal Antibody (Cat. No. bs-53112R) and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 5 \u03bcg of antibody were used per ChIP experiment. IgG (1 \u03bcg\/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ZMYND10 gene (used as a positive control) and CDC6 gene (used as a negative control). Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Catalog #1: bs-53112R

Applications: WB, ELISA, dot-blot

Reactivity: Human

Conjugate: Unconjugated