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Primary Antibodies



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Catalog #1: bs-9957R-Biotin

Applications: WB, IHC-P

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9957R-HRP

Applications: WB, IHC-P

Reactivity: Mouse, Rat

Conjugate: HRP

Catalog #1: bs-9972R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9972R-Cy3

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-9972R-Cy5

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-9972R-FITC

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: FITC

Catalog #1: bs-9972R-Biotin

Applications: WB, IHC-P

Reactivity:

Conjugate: Biotin

Catalog #1: bs-9972R-Cy7

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-9972R-HRP

Applications: WB, IHC-P

Reactivity:

Conjugate: HRP

The Jurkat\uff08Treated with PMA (25 ng\/mL, 6 h) and ionomycin (1 \u00b5g\/mL, 6 h), BFA (5 \u00b5g\/ml, last 5 h)\uff09 (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-IFN gamma antibody (bsm-0388M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488 (bs-60296G-BF488): 1 \u03bcg\/test.Blank control (black): PBS. Acquisition of 20,000 events was performed.\r\n
4% Paraformaldehyde-fixed Jurkat\uff08Treated with PMA (25 ng\/mL, 6 h) and ionomycin (1 \u00b5g\/mL, 6 h), BFA (5 \u00b5g\/ml, last 5 h)\uff09(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (IFN gamma) monoclonal Antibody, unconjugated (bsm-0388M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Catalog #1: bsm-0388m

Applications: Flow-Cyt, ICC/IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-0388m-cy3-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-0388m-cy5-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-0388m-cy7-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-0388m-fitc-100ul

Applications: Flow-Cyt

Reactivity: Human

Conjugate: FITC

Catalog #1: bsm-0388M-Biotin

Applications: WB, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-0388M-HRP

Applications: WB, IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-0940M

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded human hepatocellular carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Serum albumin (3F4) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:500 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Lane 1:Human Serum probed with Serum albumin Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (Human Serum albumin (Loading Control)) Monoclonal Antibody, Unconjugated (bsm-0945M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-0945m

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Unconjugated

Catalog #1: bsm-0945m-cy3-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy3

Catalog #1: bsm-0945m-cy5-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy5

Catalog #1: bsm-0945m-cy7-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: Cy7

Catalog #1: bsm-0945m-fitc-100ul

Applications: WB, IF(IHC-P)

Reactivity: Human, Mouse

Conjugate: FITC

Catalog #1: bsm-0945m-hrp-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: HRP

Catalog #1: bsm-0945m-biotin-100ul

Applications: WB, IHC-P

Reactivity: Human, Mouse

Conjugate: Biotin

Formalin-fixed and paraffin embedded human esophageal cancer tissue labeled with Anti-WIG 1\/PAG608 (3E4) Monoclonal Antibody, Unconjugated(bsm-0957M) at 1:200 followed by conjugation to the secondary antibody and DAB staining
293T cell lysates probed with PAG608 (3E4) Monoclonal Antibody, Unconjugated (bsm-0957M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat Cerebrum lysates probed with PAG608 (3E4) Monoclonal Antibody, Unconjugated (bsm-0957M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: 293T cell lysates probed with PAG608 (3E4) Monoclonal Antibody, Unconjugated (bsm-0957M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0957M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Rat pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TIMP-1 (4C2) Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TIMP-1 (4C2)Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TIMP-1 (4C2)Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with TIMP-1 (4C2)Monoclonal Antibody, Unconjugated (bsm-10895M) at 1:400 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-10895m

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-10895m-cy3-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-10895m-cy5-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-10895m-cy7-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-10895m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-10895m-fitc-100ul

Applications: WB, IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-10895m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-2141M

Applications: WB, ELISA, ICC/IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-2141M-Cy3

Applications: WB

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-2141M-Cy5

Applications: WB

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-2141M-Cy7

Applications: WB

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-2141M-FITC

Applications: WB

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-2141M-Biotin

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-2141M-HRP

Applications: WB, ELISA

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-2313M

Applications: WB, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-2313M-Cy3

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy3

Catalog #1: bsm-2313M-Cy5

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy5

Catalog #1: bsm-2313M-Cy7

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy7

Catalog #1: bsm-2313M-Biotin

Applications: WB, IHC-P

Reactivity:

Conjugate: Biotin

Catalog #1: bsm-2313M-FITC

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: FITC

Catalog #1: bsm-2313M-HRP

Applications: WB, IHC-P

Reactivity:

Conjugate: HRP

Lane 1: LOVO lysates; Lane 2: MCF7 lysates probed with Bcl-2 Polyclonal Antibody, Unconjugated (bs-33047M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Jurkat cell lysates; Lane 2: Hela cell lysates; Lane 3: HL60 cell lysates probed with Bcl-2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nBcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with \r\nBcl2 Monoclonal Antibody, Unconjugated (bsm-33047M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33047M

Applications: WB, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

HeLa cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
HepG2 cell lysates probed with HSP70 (5G10) Monoclonal Antibody, unconjugated (bsm-33048M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin-embedded Mouse Testis tissue; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20 minutes; Antibody incubation with HSP70 (5G10) Monoclonal Antibody, Unconjugated (bsm-33048M) at 1:400 overnight at 4\u00b0C, followed by incubation with a conjugated secondary antibody, DAB and counterstaining.

Catalog #1: bsm-33048M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Pig

Conjugate: Unconjugated