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Primary Antibodies

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Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated (bs-3195R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Human heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human gastric carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IRF3 (Ser396) Polyclonal Antibody, Unconjugated (bs-3195R) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #: bs-3195R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Unconjugated

Catalog #: bs-3195R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Biotin

Catalog #: bs-3195R-A350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-3195R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: HRP

Catalog #: bs-3195R-A594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-3195R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cyanine 7

Catalog #: bs-3195R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cyanine 3

Catalog #: bs-3195R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: FITC

Catalog #: bs-3195R-A488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-3195R-A647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-3195R-A555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-3195R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cyanine 5

Catalog #: bs-3195R-A680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-3195R-A750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human kidney labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart lysates probed with IRF3 Polyclonal Antibody, Unconjugated (bs-2993R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bs-2993R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Unconjugated

Catalog #: bs-2993R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Biotin

Catalog #: bs-2993R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: HRP

Catalog #: bs-2993R-A488

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-2993R-A555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-2993R-A350

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-2993R-A594

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-2993R-A647

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-2993R-Cy3

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Cyanine 3

Catalog #: bs-2993R-Cy5

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Cyanine 5

Catalog #: bs-2993R-Cy7

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: Cyanine 7

Catalog #: bs-2993R-FITC

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: FITC

Catalog #: bs-2993R-A680

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-2993R-A750

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Monkey

Conjugate: ALEXA FLUOR® 750

Catalog #: bs-1447R-A594

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 594

Formalin-fixed and paraffin embedded human kidney cancer labeled with Anti-HIF-2\u03b1 Polyclonal Antibody, Unconjugated (bs-1447R) followed by conjugation to the secondary antibody and DAB staining
Rat lung lysates probed with Rabbit Anti-HIF2 alpha Polyclonal Antibody, Unconjugated (bs-1447R) at 1:200 and Mouse Anti-GAPDH (bsm-0978M) at 1:500 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.
HepG2 cells(black) were fixed with 70% ice-cold methanol overnight at 4\u2103,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HIF2 alpha Polyclonal Antibody(bs-1447R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #: bs-1447R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Unconjugated

Catalog #: bs-1447R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: HRP

Catalog #: bs-1447R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Biotin

Catalog #: bs-1447R-A350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-1447R-A488

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-1447R-A555

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-1447R-A647

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-1447R-Cy3

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cyanine 3

Catalog #: bs-1447R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cyanine 5

Catalog #: bs-1447R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: Cyanine 7

Catalog #: bs-1447R-A680

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 680

Catalog #: bs-1447R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: FITC

Catalog #: bs-1447R-A750

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Sheep, Monkey

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated(bs-0081R) 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rabbit pancreas tissue labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) 1:300 followed by conjugation to the secondary antibody and DAB staining
Rat lung lysate 30ug, probed (bs-0081R) at 1:200 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted and observed band size: 17kDa,33kD
Formalin-fixed and paraffin embedded Human oral squamous cell carcinoma labeled with Anti-Caspase-3 Polyclonal Antibody, Unconjugated (bs-0081R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37\u00b0C and DAPI staining

Catalog #: bs-0081R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Unconjugated

Catalog #: bs-0081R-A350

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-0081R-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: HRP

Catalog #: bs-0081R-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Biotin

Catalog #: bs-0081R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: Cyanine 7

Catalog #: bs-0081R-A555

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Chicken, Sheep, Rabbit, Others, Monkey

Conjugate: ALEXA FLUOR® 555