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Primary Antibodies

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Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37°C, followed by a conjugated secondary antibody and DAB staining.

Catalog #: bsm-51412M

Applications: WB, IHC-P

Reactivity: Human, Monkey

Conjugate: Unconjugated

Lane 1: Hela; Lane 2: PC12; Lane 3: K562 lysate probed with JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody (bsm-52258R) overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
IF(ICC) staining with JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody (bsm-52258R) at 1:100 in NIH/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Catalog #: bsm-52258R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52262R

Applications: WB, FCM, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish

Conjugate: Unconjugated

Catalog #: bsm-52346R

Applications: WB, IHC

Reactivity: Human, Monkey

Conjugate: Unconjugated

Catalog #: bsm-52846R

Applications: WB

Reactivity: Human, Mouse, Rat, Plant, Monkey, Zebrafish, Hamster

Conjugate: Unconjugated