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Primary Antibodies

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Catalog #1: bs-6031R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-6031R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-6031R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-6031R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: Cy7

Catalog #1: bs-6031R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Monkey

Conjugate: FITC

Catalog #1: bs-0756R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0756R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Pig, Others, Monkey

Conjugate: Cy5

Lane 1: Hela; Lane 2: PC12; Lane 3: K562 lysate probed with JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody (bsm-52258R) overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with JNK1+JNK2+JNK3 (3G7) Monoclonal Antibody (bsm-52258R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52258R

Applications: WB, IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey, Zebrafish

Conjugate: Unconjugated

Catalog #1: bsm-52846R

Applications: WB

Reactivity: Human, Mouse, Rat, Plant, Monkey, Zebrafish, Hamster

Conjugate: Unconjugated

Catalog #1: bs-0179R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-1299R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-1299R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-1299R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: FITC

Catalog #1: bs-3261R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy3

Catalog #1: bs-3261R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy7

Catalog #1: bs-3261R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: FITC

Catalog #1: bs-8016R-Biotin

Applications: WB, ELISA

Reactivity: Mouse, Monkey, Macaque Monkey

Conjugate: Biotin

Catalog #1: bs-8016R-HRP

Applications: WB, ELISA

Reactivity: Mouse, Monkey, Macaque Monkey

Conjugate: HRP

Mouse bone lysates probed with Rabbit Anti-CLN3 Polyclonal Antibody, Unconjugated (bs-8016R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Mouse heart lysates probed with CLN3 Polyclonal Antibody, Unconjugated (bs-8016R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-8016R

Applications: WB, ELISA

Reactivity: Mouse, Monkey, Macaque Monkey

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (bs-8204R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (D4S234E) Polyclonal Antibody, Unconjugated (bs-8204R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Catalog #1: bs-8204R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: Unconjugated

Catalog #1: bs-8204R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Monkey

Conjugate: Biotin

Catalog #1: bs-8204R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Mouse, Monkey

Conjugate: HRP

Catalog #1: bs-8204R-A350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-8204R-A488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-8204R-A555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-8204R-Cy3

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: Cy3

Catalog #1: bs-8204R-A647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-8204R-A594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-8204R-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: Cy5

Catalog #1: bs-8204R-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: Cy7

Catalog #1: bs-8204R-FITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: FITC

Catalog #1: bs-8204R-A680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-8204R-A750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Monkey

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-0978M-Biotin

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Biotin

Catalog #1: bsm-0978M-HRP

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: HRP

Human HepG2 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Human A549 cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: mouse placenta; Lane 2: 293T cells probed with GAPDH(3E12) Polyclonal Antibody, unconjugated (bsm-0978M) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Lane 1: A549 cell lysates; Lane 2: 293T cell lysates; Lane 3: HepG2 cell lysates; Lane 4: Hela cell lysates probed with GAPDH(3E12) Monoclonal Antibody, Unconjugated (bsm-0978M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0978M

Applications: WB

Reactivity: Human, Mouse, Rat, Sheep, Rabbit, Monkey

Conjugate: Unconjugated

Formalin-fixed and paraffin embedded human kidney labeled with Anti-LIN9 Polyclonal Antibody, Unconjugated (bs-18283R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.\\n
Mouse bone lysates probed with Anti-LIN9 Polyclonal Antibody, Unconjugated (bs-18283R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:10000 for 90 min at 37˚C.\\n

Catalog #1: bs-18283R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Monkey

Conjugate: Unconjugated

Catalog #1: bs-18283R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Monkey

Conjugate: Biotin

Catalog #1: bs-18283R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Monkey

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded Rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with YY1 (3C6) Monoclonal Antibody (bsm-51412M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
Lane 1: Daudi cell lysates; Lane 2: U-251 cell lysates; probed with YY1 (3C6) Polyclonal Antibody, unconjugated (bsm-51412M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Paraformaldehyde-fixed, paraffin embedded Monkey brain tissue; Blocked with 3% BSA for 30min at RT; Antigen retrieval via heat mediation with a citrate buffer (pH6); Antibody incubation with YY1 (3C6) Monoclonal Antibody, Unconjugated (bsm-51412M) at 1:25 for 1hr at 37\u00b0C, followed by a conjugated secondary antibody and DAB staining.

Catalog #1: bsm-51412M

Applications: WB, FCM, IHC-P

Reactivity: Human, Rat, Monkey

Conjugate: Unconjugated

Western blot analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with ER alpha (5G7) Monoclonal Antibody (bsm-53007M) at a concentration of 7 \u03bcg\/ ml. Figure shows that the antibody is specific for ER alpha isoform, whereas the ER beta isoform is not recognized.
COS-7 cells transiently over-expressing human ER\u03b1 (left) or ER\u00df1 (right) were labeled with the ER alpha (5G7) Monoclonal Antibody (bsm-53007M), used at a concentration of 15 \u03bcg\/ml, followed by a biotinylated secondary antibody and peroxidase-labeled avidin. Figure shows the specificity of the antibody for the ER alpha isoform.
ChIP assays was performed using MCF7 cells treated with the ER agonist estradiol for 3 hours prior to harvesting, ER alpha (5G7) Monoclonal Antibody (bsm-53007M), and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 \u03bcg of antibody were used per ChIP experiment. Recovery (%: ChIP\/input) and occupancy (x fold: +ve\/-ve) are shown. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +\/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.

Catalog #1: bsm-53007M

Applications: WB, IHC-P, IHC, dot-blot

Reactivity: Human, Monkey

Conjugate: Unconjugated

Catalog #1: bs-17711R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Plant, Monkey, Cynomolgus Monkey

Conjugate: HRP

Catalog #1: bs-17711R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Plant, Monkey, Cynomolgus Monkey

Conjugate: Biotin

Hela lysates probed with MON1A Polyclonal Antibody, Unconjugated (bs-17711R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human 293T cell lysates; Lane 2: Human Hela cell lysates probed with MON1A Polyclonal Antibody, Unconjugated (bs-17711R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-17711R

Applications: WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Plant, Monkey, Cynomolgus Monkey

Conjugate: Unconjugated

Lane 1: NCCIT; Lane 2: PC12; Lane 3: F9; Probed with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1\/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
IF(ICC) staining with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100 in A549 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with GAPDH (3B5) Monoclonal Antibody (bsm-52262R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Catalog #1: bsm-52262R

Applications: WB, FCM, IHC-P, IF(ICC), IHC

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish

Conjugate: Unconjugated

Raji cell lysates probed with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
Paraformaldehyde-fixed and paraffin-embedded Human spleen tissue incubated with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with CD20 (2C12) Monoclonal Antibody (bsm-52346R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Catalog #1: bsm-52346R

Applications: WB, IHC-P, IHC

Reactivity: Human, Monkey

Conjugate: Unconjugated

Catalog #1: bs-1299R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse, Rat, Monkey

Conjugate: Cy5.5

Catalog #1: bs-3195R-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Monkey

Conjugate: Cy5.5