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Primary Antibodies

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Formalin-fixed and paraffin embedded: human rectal carcinoma labeled with Anti-14-3-3 Polyclonal Antibody (bs-0237R, Unconjugated) at 1:200,, followed by conjugation to the secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) at 1:200 for 40 minutes at 37°C
L1 mouse brain lysates L2 mouse kidney lysates probed with Anti 14-3-3(Alpha/Beta/Gamma/Delta/Epsilon) Polyclonal Antibody, Unconjugated (bs-0237R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C. Predicted band 31kD. Observed band size: 31kD.

Catalog #: bs-0237R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with 14-3-3 beta Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with 14-3-3 beta Polyclonal Antibody, Unconjugated (bs-12420R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Catalog #: bs-12420R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bs-50553R

Applications: WB

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bs-12420R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-12420R-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-12420R-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-12420R-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-12420R-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-12420R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #: bs-12420R-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #: bs-12420R-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #: bs-12420R-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #: bs-12420R-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #: bs-12420R-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #: bs-12420R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #: bs-12421R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bs-12421R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-12421R-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-12421R-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-12421R-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-12421R-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-12421R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #: bs-12421R-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #: bs-12421R-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #: bs-12421R-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #: bs-12421R-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #: bs-12421R-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #: bs-12421R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Formalin-fixed and paraffin embedded human breast cancer labeled with Rabbit Anti-14-3-3 protein zeta/delta (Ser58) Polyclonal Antibody, Unconjugated (bs-3000R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\\n
HepG2 cell lysates probed with 14-3-3 protein zeta (Ser58) Polyclonal Antibody, Unconjugated (bs-3000R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #: bs-3000R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bs-3000R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-3000R-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-3000R-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-3000R-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-3000R-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-3000R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #: bs-3000R-Cy3

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #: bs-3000R-Cy5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #: bs-3000R-Cy5.5

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #: bs-3000R-Cy7

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #: bs-3000R-FITC

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #: bs-3000R-HRP

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #: bs-12422R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #: bs-12422R-A350

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #: bs-12422R-A488

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #: bs-12422R-A555

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #: bs-12422R-A594

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #: bs-12422R-A647

Applications: IF(IHC-P)

Reactivity: Human, Mouse, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #: bs-12422R-Biotin

Applications: WB, IHC-P

Reactivity: Human, Mouse, Rat

Conjugate: Biotin