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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Antigen: bs-8587P, 0.2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\\nTMB staining;\\nRead the data in MicroplateReader by 450
Human HepG2 cells probed with Rabbit Anti-DOK5 Polyclonal Antibody (bs-8587R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC Conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange)

Catalog #1: bs-8587R

Applications: ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-8587R-Cy5

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-8587R-Cy7

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: Cy7

Catalog #1: bs-8587R-FITC

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-8587R-PE

Applications: FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human

Conjugate: PE

Catalog #1: bs-8595R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bs-8595R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Antigen: bs-8595P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Formalin-fixed and paraffin embedded rat brain with labeled Anti-C9orf72 Polyclonal Antibody, Unconjugated (bs-8595R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
293T cell lysates probed with C9orf72 Polyclonal Antibody, Unconjugated (bs-8595R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with C9orf72 Polyclonal Antibody(bs-8595R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Catalog #1: bs-8595R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-8595R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bs-8595R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bs-8595R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bs-8595R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bs-8605R

Applications: ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Virus, Others

Conjugate: Unconjugated

Catalog #1: bs-8634R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-8634R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Antigen: bs-8634P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Lane 1: mouse intestine lysates Lane 2: mouse pancreas lysates probed with Anti FOXA1\/HNF3-alpha Polyclonal Antibody, Unconjugated (bs-8634R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 49kD. Observed band size: 49kD.

Catalog #1: bs-8634R

Applications: WB, ELISA, IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-8876R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Bacteria, Others

Conjugate: Biotin

Catalog #1: bs-8876R

Applications: WB, ELISA, IHC-P, IHC-F, IF, ICC/IF

Reactivity: Bacteria, Others

Conjugate: Unconjugated

Catalog #1: bs-8876R-Cy3

Applications: WB, IF

Reactivity: Bacteria, Others

Conjugate: Cy3

Catalog #1: bs-8876R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Bacteria, Others

Conjugate: HRP

Catalog #1: bs-8876R-Cy5

Applications: WB, IF

Reactivity: Bacteria, Others

Conjugate: Cy5

Catalog #1: bs-8876R-Cy7

Applications: WB, IF

Reactivity: Bacteria, Others

Conjugate: Cy7

Catalog #1: bs-8876R-FITC

Applications: WB, IF

Reactivity: Bacteria, Others

Conjugate: FITC

Catalog #1: bs-9032R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity:

Conjugate: Biotin

Catalog #1: bs-9032R-HRP

Applications: WB, ELISA, IHC-P

Reactivity:

Conjugate: HRP

Catalog #1: bs-9032R

Applications: WB, ELISA, IHC-P, IF(IHC-P)

Reactivity:

Conjugate: Unconjugated

Catalog #1: bs-9032R-Cy3

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy3

Catalog #1: bs-9032R-Cy5

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy5

Catalog #1: bs-9032R-Cy7

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: Cy7

Catalog #1: bs-9032R-FITC

Applications: WB, IF(IHC-P)

Reactivity:

Conjugate: FITC

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti AMPK gamma 1\/2\/3 Polyclonal Antibody, Unconjugated (bs-9446R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-9446P, 2ug\/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; TMB staining; Read the data in Microplate Reader by 450nm.
Lane 1: mouse embryo lysates Lane 2: mouse brain lysates probed with Anti AMPK gamma 1\/2\/3 Polyclonal Antibody, Unconjugated (bs-9446R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 63kD. Observed band size: 63kD.
Formalin-fixed and paraffin embedded labeled mouse placenta with AMPK gamma 1\/2\/3 Polyclonal Antibody, Unconjugated (bs-94436R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Catalog #1: bs-9446R

Applications: WB, IHC-P, IF(IHC-P)

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bs-9446R-Biotin

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bs-9446R-HRP

Applications: WB, IHC-P

Reactivity: Rat

Conjugate: HRP

Catalog #1: bs-9446R-Cy3

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy3

Catalog #1: bs-9446R-Cy5

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy5

Catalog #1: bs-9446R-Cy7

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: Cy7

Catalog #1: bs-9446R-FITC

Applications: WB, IF(IHC-P)

Reactivity: Rat

Conjugate: FITC

Rat lung lysate 30ug, probed (bs-0061R) at 1:20000 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.
Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent localization of beta actin in mouse squamous cell cancer (KLN-205 cell line) with BS-0061R (1:50) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
A549 lysates (30ug) probed with different dilutions of beta Actin Polyclonal Antibody (bs-0061R), from 1:500-10,000 dilution and incubated overnight at 4˚C. Followed by IRDye800CW Goat Anti-Rabbit IgG at 1\/20000 dilution and 60 min incubation at 37˚C.
Lane 1: Mouse lung lysates;Lane 2: Mouse uterus lysates; Lane 3: Mouse small intestine lysates probed with beta Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-0061r

Applications: WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF

Reactivity: Human, Mouse, Rat, Hamster

Conjugate: Unconjugated

Catalog #1: bs-9861R-Biotin

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bs-9861R-HRP

Applications: ELISA, IHC-P

Reactivity: Human

Conjugate: HRP

Antigen: bs-9861P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
U2OS cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with PBST for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CMD1G Antibody\uff08bs-9861R\uff09 at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Molt4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103 and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Titin Polyclonal Antibody(bs-9861R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Mouse Muscle cell lysates; Lane 2: Rat Muscle cell lysates probed with Titin Polyclonal Antibody, Unconjugated (bs-9861R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bs-9861R

Applications: ELISA, FCM, IHC-P, IF(IHC-P)

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bs-9862R-Biotin

Applications: ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: Biotin

Catalog #1: bs-9861R-Cy3

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy3

Catalog #1: bs-9861R-Cy5

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy5

Catalog #1: bs-9861R-FITC

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: FITC

Catalog #1: bs-9861R-Cy7

Applications: FCM, IF(IHC-P)

Reactivity: Human

Conjugate: Cy7

Antigen: bs-9862P, 2ug\/100ul \\nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \\nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \\nTMB staining\\nRead the data in Microplate Reader by 450nm. \\n
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (bs-9862R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (bs-9862R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (MYH7) Polyclonal Antibody, Unconjugated (bs-9862R) at 1:200 overnight at 4\u00b0C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

Catalog #1: bs-9862R

Applications: ELISA, IHC-P, IF(IHC-P)

Reactivity: Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bs-9862R-HRP

Applications: ELISA, IHC-P

Reactivity: Mouse, Rat

Conjugate: HRP