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Primary Antibodies

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Image provided by Independent Validation Program, badge 29801.\\n\\nLane 1: HeLa G2\/M Phase cell lysate. Lane 2: C6\/36 cell lysates probed with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Antibody, Unconjugated (bs-3332R) at 1:200 for 10 hours at 4˚C. Followed by conjugation to secondary antibody at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Rabbit Anti-PI3 kinase p85 alpha + gamma (Tyr467+Tyr199) Polyclonal Antibody, Unconjugated (bs-3332R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining

Catalog #1: bs-3332R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-3332R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-3332R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Biotin

Catalog #1: bs-3332R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-3332R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-3332R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: HRP

Catalog #1: bs-3332R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-3332R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-3332R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy3

Catalog #1: bs-3332R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy5

Catalog #1: bs-3332R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-3332R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: PE

Catalog #1: bs-3332R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: Cy7

Catalog #1: bs-3332R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: FITC

Catalog #1: bs-3332R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded mouse lung labeled with (bs-0812R) Rabbit Anti-IL-1 Beta\/IL-1B Polyclonal Antibody, Unconjugated followed by conjugation to the secondary antibody and DAB staining.
Image was kindly submitted by Dr.Rachel Reith, NIH. Paraffin-embedded mouse brain stained with Rabbit Anti-IL-1 Beta Polyclonal Antibody,1:100 overnight at 4C.\\n
Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin-embedded Human Cervical Carcinoma; Antigen retrieval by boiling in sodium citrate buffer (0.01M, pH 6.0) for 15min; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-1459R) at 1:200 overnight at 4\u00b0C. Followed by incubation with the secondary antibody, DAB and counterstaining.

Catalog #1: bs-0812R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), dot-blot

Reactivity: Human, Mouse, Rat, Bovine, Pig, Rabbit, Others

Conjugate: Unconjugated

Catalog #1: bs-0061R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy5

Catalog #1: bs-0061R-HRP

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: HRP

Catalog #1: bs-0061R-Biotin

Applications: WB, ELISA, IHC-P

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Biotin

Catalog #1: bs-0061R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0061R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0061R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-0061R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-0061R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0061R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy3

Catalog #1: bs-0061R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 680

Catalog #1: bs-0061R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: Cy7

Catalog #1: bs-0061R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: FITC

Catalog #1: bs-0061R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep, Rabbit, Goat, Others, All

Conjugate: ALEXA FLUOR® 750

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) 1:300 followed by conjugation to the secondary antibody and DAB staining
L1 mouse brain lysates L2 mouse lung lysates probed with Anti Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 26kD. Observed band size:26kD.
Image kindly submitted by an end-user via Funakoshi. 4% Paraformaldehyde fixed HeLa cells stained with Rabbit Anti-Cytochrome C Polyclonal Antibody (bs-0013R) at 1:100 for 2 hours at room temperature
HepG2 cells probed with Cytochrome C Polyclonal Antibody, unconjugated (bs-0013) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange).

Catalog #1: bs-0013R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Unconjugated

Catalog #1: bs-0013R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: HRP

Catalog #1: bs-0013R-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Biotin

Catalog #1: bs-0013R-Cy3

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Cy3

Catalog #1: bs-0013R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0013R-A555

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 555

Catalog #1: bs-0013R-A594

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 594

Catalog #1: bs-0013R-A647

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 647

Catalog #1: bs-0013R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 350

Catalog #1: bs-0013R-PE

Applications: WB, FCM

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: PE

Catalog #1: bs-0013R-Cy5

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Cy5

Catalog #1: bs-0013R-Cy7

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: Cy7

Catalog #1: bs-0013R-FITC

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: FITC

Catalog #1: bs-0013R-A750

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 750

Catalog #1: bs-0013R-A680

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Chicken, Rabbit

Conjugate: ALEXA FLUOR® 680

Formalin-fixed and paraffin embedded: rabbit carotid artery labeled with Anti-TGF-beta-R2\/TGFBR2 Polyclonal Antibody (bs-0117R), Unconjugated 1:600 followed by conjugation to the secondary antibody and DAB staining
HepG2 cell lysates probed with Rabbit Anti-TGF beta R2 Polyclonal Antibody, Unconjugated (bs-0117R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.

Catalog #1: bs-0117R

Applications: WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Cow

Conjugate: Unconjugated

Catalog #1: bs-0117R-A488

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Cow

Conjugate: ALEXA FLUOR® 488

Catalog #1: bs-0117R-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat, Rabbit, Cow

Conjugate: HRP

Catalog #1: bs-0117R-A350

Applications: WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Rat, Rabbit, Cow

Conjugate: ALEXA FLUOR® 350