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Primary Antibodies

Primary Antibodies

Our Primary Antibodies are research-use antibodies designed to support protein detection and target analysis across a wide range of applications and species. Our collection includes recombinant antibodies, monoclonal antibodies, and polyclonal antibodies for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), flow cytometry (FC), ELISA, and other common applications.

These primary antibodies are available for targets involved in cancer research, immunology, neuroscience, cell biology, metabolism, apoptosis, signaling pathways, and disease-related research. Bioss offers primary antibodies guaranteed for use in human, mouse, rat, and other species to help researchers choose the right antibody format for their experimental model and detection method.



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Catalog #1: bsm-33006M-Biotin

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-41267M-HRP

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: HRP

Lane 1, 3, 5: SARS-CoV-2 N protein (Cat# bs-41417P); Lane 2, 4, 6: SARS-CoV N protein (Cat# bs-49002P); probed with SARS-CoV-2 (1G4) Nucleocapsid protein Monoclonal Antibody, Unconjugated (bsm-41267M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Recombinant SARS-CoV-2 N protein (WT) (His Tag) (bs-41408P); Lane 2: Recombinant SARS-CoV-2 N protein (Q9H, P67S, P80R, P151L, S183Y) (His Tag) (bs-41451P); Lane 3: Recombinant SARS-CoV-2 N protein (Del204, Del215) (His Tag) (bs-41491P); Lane 4: Recombinant SARS-Cov-2 (Omicron, B.1.1.529) N protein (P13L, E31del, R32del, S33del, R203K, G204R) (N-His Tag) (bs-41494P) probed with SARS-CoV-2 (2019-nCoV) Nucleocapsid Monoclonal Antibody, Unconjugated (bsm-41267M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-41267m-100ul

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41268m-100ul

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Unconjugated

Catalog #1: bsm-41268M-Biotin

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: Biotin

Catalog #1: bsm-41268M-HRP

Applications: WB, ELISA

Reactivity: SARS-CoV-2

Conjugate: HRP

Catalog #1: bsm-41269m-100ul

Applications: ELISA

Reactivity:

Conjugate: Unconjugated

Catalog #1: bsm-0391M-HRP

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-0391M-Biotin

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CRP (C11F2) Monoclonal Antibody, Unconjugated (bsm-0391M) at 1:400 overnight at 4\u00b0C, followed secondary and DAB staining.
Lane 1: C Reactive Protein (CRP); 40ug loaded into the lane; Probed with CRP (C11F2) Monoclonal Antibody, unconjugated (bsm-0391M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: C Reactive Protein(CRP) probed with CRP(C11F2) Monoclona Antibody, Unconjugated (bsm-0391M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human U-2OS cell lysates; Lane 2: Human Siha cell lysates probed with CRP(C11F2) Monoclonal Antibody, Unconjugated bsm-0391MR) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-0391M

Applications: WB, IHC-P, IHC-F, IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:400 overnight at 4\u00b0C, DAB staining.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (bsm-10697M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Human lung squamous cell carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with ICAM1\/CD54 Monoclonal Antibody, Unconjugated (ascites of bsm-10697M) at 1:800 overnight at 4\u00b0C, followed by conjugation to the bs-40296G-HRP and DAB (C-0010) staining.

Catalog #1: bsm-10697m

Applications: WB, ELISA, IHC-P, IHC-F, IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-10697m-biotin-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-10697m-hrp-100ul

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33195M-HRP

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33195M-Biotin

Applications: WB, ELISA, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Jurkat lysates probed with Survivin Polyclonal Antibody, Unconjugated (bs-33195M) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Human MOLT-4 cell lysates; Lane 2: Human U937 cell lysates probed with Survivin Monoclonal Antibody, Unconjugated (bsm-33195M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MDA-MB-231 cell lysates; Lane 2: Human Jurkat cell lysates; Lane 3: Human Raji cell lysates; Lane 4: Human Hela cell lysates; Lane 5: Human MOLT-4 cell lysates; Lane 6: Human U-2OS cell lysates probed with Survivin Monoclonal Antibody, Unconjugated (bsm-33195M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Catalog #1: bsm-33195M

Applications: WB, ELISA, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33195M-Cy3

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33195M-Cy5

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33195M-Cy5.5

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33195M-FITC

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: FITC

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LC3A Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (LC3A ) Monoclonal Antibody, Unconjugated (bsm-33309M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33309m

Applications: WB, IHC-P, IHC-F, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33309m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: Biotin

Catalog #1: bsm-33309m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Mouse, Rat

Conjugate: HRP

Catalog #1: bsm-33309m-Cy5.5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33309m-cy3-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy3

Catalog #1: bsm-33309m-cy5-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy5

Catalog #1: bsm-33309m-cy7-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: Cy7

Catalog #1: bsm-33309m-fitc-100ul

Applications: WB, IF(ICC), IF

Reactivity: Human, Mouse, Rat

Conjugate: FITC

Catalog #1: bsm-33195M-Cy7

Applications: WB, IF(ICC), IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33007M-Biotin

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Biotin

Catalog #1: bsm-33007M-HRP

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: HRP

GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein Overexpression E.coli Lysates probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:2000~1:10000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST-Tagged fusion protein probed with GST tag Monoclonal Antibody, Unconjugated (bsm-33007M) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
GST tag was immunoprecipitated from GST-tagged mouse S100A7 expression vector whole cell lysate with bsm-33007M at 1\/150 dilution. Western blot was performed from the immunoprecipitate using protein A\/G beads. HRP conjugated Goat Anti-Mouse IgG (H&L) was used as secondary antibody.\r\n\r\nLane 1: GST-tagged mouse S100A7 expression vector whole cell lysate 5 \u00b5g (Input).\r\nLane 2: bsm-33007M IP in GST-tagged mouse S100A7 expression vector whole cell lysate.\r\nLane 3: native mouse IgG IP in GST-tagged mouse S100A7 expression vector whole cell lysate (negative control).\r\n

Catalog #1: bsm-33007m-100ul

Applications: WB, ELISA

Reactivity: Species independent

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with C-erbB-2\/HER2(13H9) Monoclonal Antibody, Unconjugated (bsm-33051M) at 1:800 overnight at 4\u00b0C, DAB staining.
25 ug total protein per lane of various lysates (see on figure) probed with C-erbB-2\/HER2 monoclonal antibody, unconjugated (bsm-33051M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Catalog #1: bsm-33051m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33051m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33051m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Catalog #1: bsm-33051m-cy3-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy3

Catalog #1: bsm-33051m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy5

Catalog #1: bsm-33051m-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy5.5

Catalog #1: bsm-33051m-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: Cy7

Catalog #1: bsm-33051m-fitc-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human

Conjugate: FITC

25 ug total protein per lane of various lysates (see on figure) probed with Cytokeratin 19 monoclonal antibody, Biotin conjugated (bsm-33057M-Bio) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by HRP conjugated Streptavidin (bs-0437P-HRP) incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M-Bio) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0437P-HRP and DAB (C-0010) staining.

Catalog #1: bsm-33057m-biotin-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Paraformaldehyde-fixed, paraffin embedded Human stomach cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cytokeratin 19 Monoclonal Antibody, Unconjugated (bsm-33057M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (bsm-33057M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat bladder\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (bsm-33057M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma\uff09; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Cytokeratin 19) Monoclonal Antibody, Unconjugated (bsm-33057M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33057m

Applications: WB, IHC-P, IHC-F, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33057m-hrp-100ul

Applications: WB, IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Catalog #1: bsm-33057m-cy3-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33057m-cy5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33057m-Cy5.5-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33057m-cy7-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33057m-fitc-100ul

Applications: WB, IF(IHC-P), IF(ICC), IF

Reactivity: Human, Rat

Conjugate: FITC