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Primary Antibodies

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Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (bsm-33297M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Actin, alpha skeletal muscle) Monoclonal Antibody, Unconjugated (bsm-33297M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33297M

Applications: IHC-P, IHC-F, IF, ICC

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33297M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33297M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (ATG5) Monoclonal Antibody, Unconjugated (ascites of bsm-33385M 5D7) at 1:2000 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Lane 1: Human Hela cell lysates; Lane 2: Human Panc-1 cell lysates; Lane 3: Human U87MG cell lysates; Lane 4: Human MCF-7 cell lysates; Lane 5: Human K562 cell lysates; Lane 6: Human SH-SY5Y cell lysates probed with ATG5\/APG5L Monoclonal Antibody, Unconjugated (bsm-33385M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (ATG5\/APG5L) monoclonal Antibody, Unconjugated (bsm-33385M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (XXX) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.) polyclonal Antibody, Unconjugated (bsm-33385M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Catalog #1: bsm-33385M

Applications: IHC-P, IHC-F, IF, ICC

Reactivity: Rat

Conjugate: Unconjugated

Catalog #1: bsm-33385M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Rat

Conjugate: Biotin

Catalog #1: bsm-33385M-HRP

Applications: IHC-P, IHC-F

Reactivity: Rat

Conjugate: HRP

Catalog #1: bsm-41540M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41539M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Catalog #1: bsm-41541M

Applications: ELISA

Reactivity: Virus

Conjugate: Unconjugated

Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ki-67 Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Ki-67 Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:400 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human cecum cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with CK7 Monoclonal Antibody, Unconjugated (bsm-33070M) at 1:200 overnight at 4\u00b0C, DAB staining.

Catalog #1: bsm-33070M

Applications: FCM, IHC-P, IHC-F, IF

Reactivity: Human, Rat

Conjugate: Unconjugated

Catalog #1: bsm-33070M-A488

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 488

Catalog #1: bsm-33070M-A350

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 350

Catalog #1: bsm-33070M-A647

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 647

Catalog #1: bsm-33070M-A594

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 594

Catalog #1: bsm-33070M-A555

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 555

Catalog #1: bsm-33070M-Cy5

Applications: FCM

Reactivity: Human, Rat

Conjugate: Cy5

Catalog #1: bsm-33070M-Cy5.5

Applications: FCM

Reactivity: Human, Rat

Conjugate: Cy5.5

Catalog #1: bsm-33070M-Cy3

Applications: FCM

Reactivity: Human, Rat

Conjugate: Cy3

Catalog #1: bsm-33070M-FITC

Applications: FCM

Reactivity: Human, Rat

Conjugate: FITC

Catalog #1: bsm-33070M-Cy7

Applications: FCM

Reactivity: Human, Rat

Conjugate: Cy7

Catalog #1: bsm-33070M-A750

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 750

Catalog #1: bsm-33070M-PE

Applications: FCM

Reactivity: Human, Rat

Conjugate: PE

Catalog #1: bsm-33070M-Biotin

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: Biotin

Catalog #1: bsm-33070M-A680

Applications: FCM

Reactivity: Human, Rat

Conjugate: ALEXA FLUOR® 680

Catalog #1: bsm-33070M-HRP

Applications: IHC-P, IHC-F

Reactivity: Human, Rat

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (bsm-33860M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (bsm-33860M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8+A9 Complex) Monoclonal Antibody, Unconjugated (bsm-33860M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33860M

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33860M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33860M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8) Monoclonal Antibody, Unconjugated (bsm-33861M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8) Monoclonal Antibody, Unconjugated (bsm-33861M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (S100A8) Monoclonal Antibody, Unconjugated (bsm-33861M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Catalog #1: bsm-33861M

Applications: IHC-P

Reactivity: Human

Conjugate: Unconjugated

Catalog #1: bsm-33861M-Biotin

Applications: IHC-P

Reactivity: Human

Conjugate: Biotin

Catalog #1: bsm-33861M-HRP

Applications: IHC-P

Reactivity: Human

Conjugate: HRP

Catalog #1: bs-0023M-BF555

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 555

Catalog #1: bs-0023M-BF350

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 350

Catalog #1: bs-0023M-BF405

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 405

Catalog #1: bs-0023M-BF488

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 488

Catalog #1: bs-0023M-APC-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: APC-Cy5.5

Catalog #1: bs-0023M-BF594

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 594

Catalog #1: bs-0023M-BF647

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 647

Catalog #1: bs-0023M-BF680

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 680

Catalog #1: bs-0023M-PerCP

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: PerCP

Catalog #1: bs-0023M-BF750

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: AbBy Fluor® 750

Catalog #1: bs-0023M-APC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: APC

Catalog #1: bs-0023M-RBITC

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: RBITC

Catalog #1: bs-0023M-PerCP-Cy5.5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: PerCP-Cy5.5

Catalog #1: bs-0023M-APC-Cy5

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: APC-Cy5

Catalog #1: bs-0023M-PerCP-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: PerCP-Cy7

Catalog #1: bs-0023M-APC-Cy7

Applications: WB, IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Human, Mouse, Zebrafish

Conjugate: APC-Cy7

Catalog #1: bs-0024M-BF555

Applications: IF(IHC-P), IF(IHC-F), IF(ICC)

Reactivity: Mouse

Conjugate: AbBy Fluor® 555